Establishment and characterization of an antihuman thyrotropin (TSH) receptor-specific CD4+ T cell line from a patient with Graves' disease: evidence for multiple T cell epitopes on the TSH receptor including the transmembrane domain
| Autor: | Toru Mori, Takashi Akamizu, Yoshimichi Ueda, Li Hua, Jyoji Okuda |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes endocrine system endocrine system diseases Endocrinology Diabetes and Metabolism Graves' disease T cell Molecular Sequence Data Receptors Antigen T-Cell Gene Expression Biology Cell Line T-Cell Epitopes Epitopes Endocrinology medicine Cytotoxic T cell Humans Amino Acid Sequence Receptor Cd4 t cell Cell Membrane Receptors Thyrotropin medicine.disease Flow Cytometry Molecular biology Graves Disease Peptide Fragments Transmembrane domain medicine.anatomical_structure Female CD8 |
| Zdroj: | Thyroid : official journal of the American Thyroid Association. 5(4) |
| ISSN: | 1050-7256 |
| Popis: | From the peripheral lymphocytes of a patient with Graves' disease, we established a T cell line using its reaction to a pool of 49 synthetic peptides corresponding to the entire human thyrotropin receptor (TSHR) sequence. This T cell line showed a specific response to the pool of peptides in a microproliferation assay (stimulation index: 4.8). Flow cytometry analysis revealed that the cell surface markers were CD4+ CD8-, T cell receptor (TcR) alpha beta+, and Tcr gamma delta-. To investigate T cell epitopes on TSHR, the T cell line reacted well against three groups: the N-terminal (amino acids 31-169) and C-terminal (338-420) regions of the extracellular domain and the N-terminal half (441-661) of the transmembrane domain of the receptor. This suggests a multiplicity of T cell epitopes on the TSHR, and was further supported by analysis of TcR gene expression in the cell line that showed the expression of 5 V alpha genes; V alpha-1, 2, 10, 20, and w25. In conclusion, the results of the present study indicated multiple T cell epitopes on the TSHR molecule including the transmembrane domain. |
| Databáze: | OpenAIRE |
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