Cytology vs molecular analysis for the detection of head and neck squamous cell carcinoma in oesopharyngeal brush samples: a prospective study in 56 patients
| Autor: | Bernard Luboinski, François Janot, G. Leroux, M Trassard, Stéphane Temam, Gilbert M. Lenoir, Jean Bénard, Jacques Bosq |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Esophageal Neoplasms cytodiagnosis Allelic Imbalance Biology Polymerase Chain Reaction law.invention Esophagus law Cytology Biomarkers Tumor medicine Humans Lymphocytes Prospective Studies Polymerase chain reaction DNA Primers Chromosome Aberrations Molecular and Cellular Pathology Microsatellite instability p53 gene Pharyngeal Neoplasms DNA Neoplasm medicine.disease head and neck carcinoma Head and neck squamous-cell carcinoma Oncology Epidermoid carcinoma Head and Neck Neoplasms Cytopathology Cancer cell genetic markers Carcinoma Squamous Cell Pharynx Microsatellite Tumor Suppressor Protein p53 microsatellite repeats |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6600953 |
| Popis: | Oesopharyngeal brush (OPB) sampling with cytological analysis can yield exfoliated cells from asymptomatic tumours of the upper aero-digestive tract and the oesophagus. In this study, we compared cytological evaluation and molecular analysis for the detection of exfoliated cancer cells sampled with an OPB. A total of 56 patients with a known unique head and neck squamous cell carcinoma (HNSCC) and five healthy controls were enrolled prospectively. Exfoliated cells from these 61 patients were collected with an OPB before initial endoscopy. p53 mutations and UT 5085 microsatellite instability (MI) were analysed in the HNSCC tumour, lymphocytes and the corresponding OPB DNA samples. p53 mutations and UT5085 MI were detected in 31 out of 56 and 14 out of 56 HNSCC, respectively, but not in any of the five controls. Direct sequencing of p53 was able to detect mutations in OPB DNA in only two out of 29 patients harbouring a p53-mutated primary tumour. Microsatellite instability was detected in OPB DNA of 11 out of 13 informative (bandshift detected in tumour) patients, whereas cytological analysis detected abnormal cells in only six of the same 13 patients (P=0.03). In informative patients, all positive OPB samples at cytological analysis were also positive at molecular analysis of UT5085, and both analyses confirmed the two negative samples. Molecular analysis of OPB from eight uninformative patients and from five healthy controls were all negative. OPB sampling with MI-based molecular analysis could be efficient for early detection of recurrent HNSCC. This result prompts us to use other microsatellite markers in order to maximise the percentage of informative patients. |
| Databáze: | OpenAIRE |
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