Telomere length in myelodysplastic syndromes
| Autor: | Johana Meléndez-Santiago, Alan F. List, Jeffrey E. Lancet, Jong Y. Park, Hyun Park, Jeffrey S. Painter, Pearlie K. Epling-Burnette, William J. Fulp, Rami S. Komrokji, Dana E. Rollison, Ji-Hyun Lee, Kristen A. Jonathan, Ashley L. Cole, Mayenha Guerrier |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Cancer Research Telomerase Saliva Genotype Antigens CD19 Lewis X Antigen Biology Polymerase Chain Reaction Article Young Adult Gene Frequency hemic and lymphatic diseases Paint medicine Leukocytes Humans Telomerase reverse transcriptase Pesticides In Situ Hybridization Fluorescence Aged Aged 80 and over Myelodysplastic syndromes Cancer Hematology Middle Aged Telomere medicine.disease Flow Cytometry Molecular biology Real-time polymerase chain reaction Logistic Models Oncology Case-Control Studies Myelodysplastic Syndromes Immunology Mutation Female |
| Zdroj: | Leukemialymphoma. 52(8) |
| ISSN: | 1029-2403 |
| Popis: | The relationship between telomere length (TL) and predisposition to myelodysplastic syndromes (MDS) remains unclear. We compared peripheral blood leukocyte (PBL) TL among cases of histologically confirmed MDS (n = 65) who were treatment-naive with no prior cancer history to age-matched controls (n = 63). Relative TL was measured in PBLs and saliva by quantitative polymerase chain reaction (PCR) and in CD15+ and CD19+ cells by flow cytometry-fluorescence in situ hybridization (flow-FISH). Human telomerase reverse transcriptase gene (hTERT) mutations were assessed by PCR. After adjustment for age and sex, relative TLs were reduced in PBLs (p = 0.02), CD15+ (p = 0.01), CD19+ (p = 0.25), and saliva (p = 0.13) in MDS cases versus controls, although only the PBL and CD15+ results were statistically significant. Among MDS cases, CD15+ and CD19+ cell TLs were positively correlated (p = 0.03). PBL TL was reduced among those occupationally exposed to paints and pesticides, but was not associated with hTERT genotype. Future studies are needed to further investigate constitutional telomere attrition as a possible predisposing factor for MDS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |