F. NOVICIDA-INFECTED A. CASTELLANII DOES NOT ENHANCE BACTERIAL VIRULENCE IN MICE
| Autor: | Yousef Abu Kwaik, Valentina Marečić, Marina Šantić, Martin Brezovec, Zlatko Trobonjača, Ivana Gobin, Mateja Ozanic |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) mice 030106 microbiology Immunology lcsh:QR1-502 Pathogenesis Legionella pneumophila Microbiology lcsh:Microbiology Francisella amoeba tularemia pathogenesis Tularemia 03 medical and health sciences Mice BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Medical Microbiology parasitic diseases Water environment medicine Animals Amoeba Francisella tularensis Original Research Infectivity Acanthamoeba castellanii Mice Inbred BALB C biology Virulence Intracellular parasite respiratory system biology.organism_classification medicine.disease bacterial infections and mycoses Virology Coculture Techniques 3. Good health Disease Models Animal Infectious Diseases bacteria BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Medicinska mikrobiologija |
| Zdroj: | Frontiers in Cellular and Infection Microbiology, Vol 6 (2016) Frontiers in Cellular and Infection Microbiology Volume 6 |
| ISSN: | 2235-2988 |
| Popis: | Francisella tularensis is a facultative intracellular bacterium that causes tularemia in humans and animals. Epidemiology of tularemia worldwide is often associated with water-borne transmission, which includes mosquitoes and amoebae as the potential host reservoirs of the bacteria in water environment. In vitro studies showed intracellular replication of F. tularensis within Acanthamoeba castellanii and Hartmanella vermiformis cells. While infection of amoeba by Legionella pneumophila has been shown to enhance infectivity of L. pneumophila the role of F. tularensis-infected protozoa in the pathogenesis of tularemia is not known. We used 6 h coculture of A. castellanii and F. novicida for investigation of the effect of inhaled amoeba on the pathogenesis of tularemia on in vivo model. Balb/c mice were infected intratracheally with F. novicida or with F. novicida-infected A. castellanii. Surprisingly, infection with F. novicida-infected A. castellanii did not lead to bronchopneumonia in Balb/c mice, and Francisella did not disseminate into the liver and spleen. Upon inhalation, F. novicida infects a variety of host cells, though neutrophils are the predominant cells early during infection in the lung infiltrates of pulmonary tularemia. The numbers of neutrophils in the lungs of Balb/c mice were significantly lower in the infection of mice with F. novicida-infected A. castellanii in comparison to group of mice infected only with F. novicida. These results demonstrate that following inoculation of mice with F. novicida-infected A. castellanii, mice did not develop tularemia. |
| Databáze: | OpenAIRE |
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