Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs

Autor:	M. Majibur Rahman, M. Mahbubur Rahman, Md. Shahanoor Alam, Suzan Chandra Deb, Md. Jahangir Alam, Md. Tofazzal Islam
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	0301 basic medicine food.ingredient Fish farming Science 030106 microbiology Article Enterococcus faecalis Microbiology Fish Diseases 03 medical and health sciences Sativum Antibiotic resistance food Drug Resistance Multiple Bacterial Animals Pathogen Catfishes Gram-Positive Bacterial Infections Multidisciplinary biology Plant Extracts Aquaculture of tilapia food and beverages Tilapia biology.organism_classification Medicine Catfish
Zdroj:	Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) Scientific Reports
ISSN:	2045-2322
Popis:	The opportunistic fish pathogen, Enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. The objectives of this study were to (i) identify E. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of E. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. A total of 48 isolates were phenotypically identified as Enterococcus species from tilapia, stinging catfish and walking catfish cultivated in several fish farms in Gazipur. Ten randomly selected isolates were identified as E.faecalis by 16S rRNA gene sequencing. Artificial infection revealed that most of the isolates caused moderate to high mortality in fishes with characteristic disease symptoms. These isolates exhibited resistance to multiple antibiotics in vitro. Bioassay revealed that organic extracts of Tamarindus indica and Emblica officinalis leaves, Allium sativum bulb, and Syzygium aromaticum bud inhibited the growth of E. faecalis. Methanol extracts of A. sativum and methanol and acetone extracts of S. aromaticum significantly reduced the mortality of fish artificially infected with E. faecalis as both preventive and therapeutic agents. This is the first report on molecular identification, and herbal control of fish pathogenic E. faecalis in Bangladesh.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::716750f827e439de45eb03603399c77a https://doaj.org/article/eacfa5cf7946406390db51810d3ded96 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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