Population structure in the endangered Blanca Cacerena bovine breed demonstrated by RAPD analyses
| Autor: | M Esther Sansinforiano, J.C. Parejo, M. Martínez-Trancón, J. A. Padilla, A. Rabasco |
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| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
Genetics Heterozygote Genetic diversity education.field_of_study Polymorphism Genetic Dendrogram Population Endangered species Genetic Variation General Medicine Biology Analysis of molecular variance Breed Random Amplified Polymorphic DNA Technique RAPD Genetics Population Evolutionary biology Animals Cattle Genetic variability education Molecular Biology Phylogeny |
| Zdroj: | Genes & Genetic Systems. 77:51-58 |
| ISSN: | 1880-5779 1341-7568 |
| Popis: | RAPD analyses have been used to determine the genetic diversity and the population structure of the endangered Blanca Cacereña bovine breed. Genetic variability was evaluated on the basis of 1048 loci produced by 71 primers. RAPD produced a number of polymorphic loci (30.44%), and it has been proved to be a useful method for evaluating polymorphisms in this breed. The dendrograms based on simililarity indexes and on Nei's genetic distances between 60 animals and the value of genetic differentiation among subpopulations (F(ST)) showed a clear population substructure defined by herds and a scarce genetic flow among herds. Analysis of molecular variance (AMOVA) showed that 32.4% of the total variance was due to differences among herds and confirmed the clustering found. The results of the present study allow us to plan more adequate mating in order to maintain the genetic diversity and to improve the efficiency of conservation for the Blanca Cacereña bovine breed. |
| Databáze: | OpenAIRE |
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