Signaling through CD50 (ICAM-3) stimulates T lymphocyte binding to human umbilical vein endothelial cells and extracellular matrix proteins via an increase in β1 and β2 integrin function
| Autor: | Jordi Esparza, Ramon Vilella, Agust́i Miralles, Antoni Gayà, Maria C. Cid, Jaume Ordi, Alvaro Urbano-Márquez, Jordi Vives, Manel Juan, Jordi Yagüe |
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| Rok vydání: | 1994 |
| Předmět: |
Integrins
Umbilical Veins T-Lymphocytes Immunology Intercellular Adhesion Molecule-1 Integrin Biology Extracellular matrix Cell–cell interaction Antigen Antigens CD Cell Adhesion Humans Immunology and Allergy Lymphocyte homing receptor Cells Cultured Cell Aggregation Cell adhesion molecule Integrin beta1 T lymphocyte Flow Cytometry Antigens Differentiation Molecular biology Fibronectins Cell biology CD18 Antigens biology.protein Endothelium Vascular Cell Adhesion Molecules |
| Zdroj: | European Journal of Immunology. 24:1377-1382 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830240621 |
| Popis: | Regulated adhesion of T lymphocytes to antigen-presenting cells, endothelial cells and extracellular matrix proteins is crucial in T lymphocyte activation and migration to the sites of injury. In this study, we show that three monoclonal antibodies (mAb) recognizing different epitopes on the CD50 (ICAM-3) molecule increase T lymphocyte adhesion to tumor necrosis factor (TNF)-stimulated human umbilical vein endothelial cells and extracellular matrix proteins. These phenomena are mediated by an increase in beta 1 and beta 2 integrin avidity since (a) CD50-induced adhesion to endothelial cells was abrogated by simultaneous blocking of beta 1- and beta 2-mediated adhesion pathways but not by interfering with either one individually, (b) CD50 mAb increased beta 1 integrin-mediated adhesion to extracellular matrix proteins and to fibronectin-derived synthetic peptides, (c) CD50 mAb enhanced T lymphocyte binding to ICAM-1 transfectants, and (d) CD50 mAb did not modify surface expression patterns of beta 1 or beta 2 integrins on T lymphocytes. Our data suggest that constitutively expressed CD50 (ICAM-3) can play a pivotal role in initiating a cascade of adhesion events which may be crucial in immune activation and in the development of inflammatory lesions. |
| Databáze: | OpenAIRE |
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