Role of interleukin-2 in regulating lymphocyte activation and recirculation
| Autor: | Giuseppe Di Lucca, Claudio Fortis, C. Besana, Elisabetta Ferrero, Claudio Rugarli, Consuelo Corti, Silvia Heltai, Marco Foppoli, Giuseppe Consogno |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Interleukin 2
Cancer Research medicine.medical_specialty Lymphocytosis High endothelial venules Immunofluorescence Lymphocyte Activation Peripheral blood mononuclear cell Leukocyte Count Internal medicine Medicine Humans Centrifugation Carcinoma Renal Cell medicine.diagnostic_test business.industry Extravasation Kidney Neoplasms Killer Cells Natural Endocrinology Oncology Immunology CD4 Antigens Interleukin-2 medicine.symptom business CD8 medicine.drug |
| Zdroj: | European journal of cancer (Oxford, England : 1990). (3) |
| ISSN: | 0959-8049 |
| Popis: | TO UNDERSTAND better the immunological reactions primed by interleukin-2 (IL-2) in cancer patients we have investigated the biological effects of systemic rIL-2 on the circulating lymphocytes of cancer patients [ 1, 21. We report here immunological tests, either functional or phenotypic, in 8 patients with advanced renal cell carcinoma treated with rIL-2 (18 x lo6 U/m*/day) by continuous intravenous administration for 5 consecutive days. Heparinised blood samples were drawn immediately before starting the first treatment cycle, 24 and 96 h after starting the rIL-2 infusion, and 2 days after the end of the infusion (168 h). Mononuclear cells were obtained by centrifugation over Ficoll gradient and resuspended in RPM1 1640 medium (Biochrom, Berlin, FRG). Phenotypic analysis was performed in a direct, doubie-staining, immunofluorescence assay. During lymphopenia, induced by the IL-2 administration, we observed a progressive decrease in the percentage of theCD3+CD4+ T-lymphocytes, while theCD3+CD8+ subset did not change significantly. Small CD4+ and CDS+ T cells are extracted by high endothelial venules with different efficiency (higher for the CD4+ than for the CD8+ subset) {3] and the rIL-2 infusion seems to emphasise this difference. The hypothesis that the decrease of the CD4+ T cells might be due to increased extravasation, is also sustained by concomitant reduced proliferation of the patients’ peripheral blood mononuclear cells (PBMC) cultured for 3 days with different concentrations of phytohaemaglutinin (PI-IA) or concavalin A (ConA) (Sigma Chemical Co, St. Louis, Missouri). On stopping the rIL-2 infusion, when a rebound lymphocytosis is observed, the percentage of the CD4+ T-lymphocytes, and the capacity of |
| Databáze: | OpenAIRE |
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