Microsatellite typing ofAspergillus flavusin patients with various clinical presentations of aspergillosis
| Autor: | Sourour Neji, Inès Hadrich, Houwaida Trabelsi, Fattouma Makni, Ali Ayadi, Stéphane Ranque, Nedia Mahfoud, I. Drira |
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| Rok vydání: | 2013 |
| Předmět: |
Genotype
Molecular Sequence Data Population Aspergillus flavus Aspergillosis Microbiology medicine Humans Clinical significance Typing DNA Fungal Mycological Typing Techniques education Molecular Epidemiology education.field_of_study biology Molecular epidemiology Sequence Analysis DNA General Medicine biology.organism_classification medicine.disease Molecular Typing Infectious Diseases Microsatellite Microsatellite Repeats |
| Zdroj: | Medical Mycology. 51:586-591 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.3109/13693786.2012.761359 |
| Popis: | Aspergillus flavus is the second most important Aspergillus species associated with aspergillosis and the incidence of infections caused by it are increasing in the immunocompromised population. This species is of major epidemiological importance in regions with a dry and hot climate. Despite the growing clinical significance of A. flavus, data on its molecular epidemiology are scarce. This study was aimed at examining whether isolates from distinct genotypes were involved in distinct clinical forms of aspergillosis. Sixty-three clinical isolates of A. flavus recovered from 35 patients with various clinical presentations of aspergillosis were characterized by microsatellite typing. The highest discriminatory power for a single locus was obtained with the AFLA1 marker, which had 14 distinct alleles and a 0.903 D value. The combination of all six markers yielded 48 different genotypes with a 0.994 D value. There was a considerable genetic diversity in the isolates and patients with invasive aspergillosis were usually colonized by multiples genotypes. There was no evidence that a given genotype was associated with a particular clinical presentation of A. flavus aspergillosis. The occurrence of more than one genotype in clinical samples indicates that a patient may be infected by multiple genotypes and that any particular isolate from a clinical specimen may not necessarily be the one causing aspergillosis. |
| Databáze: | OpenAIRE |
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