Mouse adult hematopoietic stem cells actively synthetize ribosomal RNA

Autor: Ana Cumano, Alexandre David, Sandrine Vandormael-Pournin, Sabrina Coqueran, Michel Cohen-Tannoudji, Marie Le Bouteiller, Aurélien Raveux, Léonard Jarzebowski
Přispěvatelé: Embryon précoce de mammifères et cellules souches, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Développement des Lymphocytes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique, and the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE). L.J. was supported by the DIM biotherapies Ile-de-France, the Société Française d'Hématologie, and the REVIVE Labex. M.L.B. was supported by the Ligue Nationale Contre le Cancer, Imaging and cell sorting analyses were performed at the Imagopole of Institut Pasteur. We are grateful to P.-H. Commere for his assistance with FACS analysis and sorting. We thank F. Morlé, L. Da Costa, and all members of the laboratory for technical advice and helpful discussions., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: RNA
RNA, 2018, 24 (12), pp.1803-1812. ⟨10.1261/rna.067843.118⟩
RNA, Cold Spring Harbor Laboratory Press, 2018, 24 (12), pp.1803-1812. ⟨10.1261/rna.067843.118⟩
ISSN: 1355-8382
1469-9001
DOI: 10.1261/rna.067843.118⟩
Popis: International audience; Contribution of basal cellular processes to the regulation of tissue homeostasis has just started to be appreciated. However, our knowledge on the modulation of ribosome biogenesis activity in situ within specific lineages remains very limited. This is largely due to the lack of assays that enable quantitation of ribosome biogenesis in small numbers of cells in vivo. We used a technique, named Flow-FISH, combining cell surface antibody staining and flow cytometry with intracellular ribosomal RNA (rRNA) FISH, to measure the levels of pre-rRNAs of hematopoietic cells in vivo. Here, we show that Flow-FISH reports and quantifies ribosome biogenesis activity in hematopoietic cell populations, thereby providing original data on this fundamental process notably in rare populations such as hematopoietic stem and progenitor cells. We unravel variations in pre-rRNA levels between different hematopoietic progenitor compartments and during erythroid differentiation. In particular, our data indicate that, contrary to what may be anticipated from their quiescent state, hematopoietic stem cells have significant ribosome biogenesis activity. Moreover, variations in pre-rRNA levels do not correlate with proliferation rate suggesting that cell type specific mechanisms might regulate ribosome biogenesis in hematopoietic stem cells and progenitors. Our study contributes to a better understanding of the cellular physiology of the hematopoietic system in vivo in unperturbed situation.
Databáze: OpenAIRE
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