INGAP-related pentadecapeptide: its modulatory effect upon insulin secretion
| Autor: | María Inés Borelli, Luiz Fabrizio Stoppiglia, Luis Emilio Flores, Luiz F. Rezende, Hector Herminio del Zotto, Juan José Gagliardino, Antonio C. Boschero |
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| Rok vydání: | 2004 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Arginine Physiology medicine.medical_treatment Clinical Biochemistry Endogeny Pancreatitis-Associated Proteins Biology Biochemistry Cellular and Molecular Neuroscience Islets of Langerhans Endocrinology Leucine Internal medicine Insulin Secretion medicine Animals Insulin Secretion Amino Acid Sequence Rats Wistar Pancreatic hormone Cells Cultured geography geography.geographical_feature_category Islet Peptide Fragments Insulin oscillation Rats Animals Newborn Liberation Cytokines Peptides |
| Zdroj: | Regulatory peptides. 131(1-3) |
| ISSN: | 0167-0115 |
| Popis: | We examined the effects of a pentadecapeptide having the 104–118 aminoacid sequence of islet neogenesis-associated protein (INGAP-PP) on insulin secretion, and the morphological characteristics of adult and neonatal pancreatic rat islets cultured in RPMI and 10 mM glucose for 4 days, with or without different INGAP-PP concentrations (0.1–100 μg/ml). A scrambled 15 aminoacid peptide was used as control for the specificity of INGAP-PP effect. Cultured neonatal and adult islets released insulin in response to glucose (2.8–16.7 mM) in a dose-dependent manner, and to leucine and arginine (10 mM). In all cases, the response was greater in adult islets. INGAP-PP added to the culture medium significantly enhanced glucose- and aminoacid-induced insulin release in both adult and newborn rats; however, no changes were observed with the scrambled peptide. Similar results were obtained incubating freshly isolated adult rat islets with INGAP-PP. Whereas INGAP-PP did not induce significant changes in islet survival rate or proportion/number of islet cells, it increased significantly β-cell size. This first demonstration of the enhancing effect of INGAP-PP on the β-cell secretory response of adult and newborn islets opens a new avenue to study its production mechanism and potential use to increase the secretory capacity of endogenous islets in intact animals or of islets preserved for future transplants. |
| Databáze: | OpenAIRE |
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