Effect of Npt2b deletion on intestinal and renal inorganic phosphate (Pi) handling

Autor: Toru Fujii, Akira Maeda, Sumire Sasaki, Sawako Tatsumi, Hiroko Segawa, Ruri Kirino, Shohei Sasaki, Yasuhiro Ichida, Miwa Noguchi, Ai Hanazaki, Aoi Kushi, Yosuke Kawase, Otoya Ueda, Ichiro Kaneko, Naoshi Horiba, Hiromi Tateishi, Kayo Ikuta, Naoko A. Wada, Mami Kakefuda, Kou-ichi Jishage, Ken-ichi Miyamoto, Shuichi Ohtomo, Yuka Kawabata
Rok vydání: 2017
Předmět:
Zdroj: Clinical and Experimental Nephrology. 22:517-528
ISSN: 1437-7799
1342-1751
Popis: Hyperphosphatemia is common in chronic kidney disease and is associated with morbidity and mortality. The intestinal Na+-dependent phosphate transporter Npt2b is thought to be an important molecular target for the prevention of hyperphosphatemia. The role of Npt2b in the net absorption of inorganic phosphate (Pi), however, is controversial. In the present study, we made tamoxifen-inducible Npt2b conditional knockout (CKO) mice to analyze systemic Pi metabolism, including intestinal Pi absorption. Although the Na+-dependent Pi transport in brush-border membrane vesicle uptake levels was significantly decreased in the distal intestine of Npt2b CKO mice compared with control mice, plasma Pi and fecal Pi excretion levels were not significantly different. Data obtained using the intestinal loop technique showed that Pi uptake in Npt2b CKO mice was not affected at a Pi concentration of 4 mM, which is considered the typical luminal Pi concentration after meals in mice. Claudin, which may be involved in paracellular pathways, as well as claudin-2, 12, and 15 protein levels were significantly decreased in the Npt2b CKO mice. Thus, Npt2b deficiency did not affect Pi absorption within the range of Pi concentrations that normally occurs after meals. These findings indicate that abnormal Pi metabolism may also be involved in tight junction molecules such as Cldns that are affected by Npt2b deficiency.
Databáze: OpenAIRE
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