A DNA-Modified Live Vaccine Prime–Boost Strategy Broadens the T-Cell Response and Enhances the Antibody Response against the Porcine Reproductive and Respiratory Syndrome Virus
| Autor: | Elise Bordet, Virginie Jakob, Cindy Bernelin-Cottet, Céline Barc, Edwige Bouguyon, Ivan Trus, Nicolas Collin, Olivier Boulesteix, Christophe Barnier-Quer, Elisabeth Stubsrud, Céline Urien, Isabelle Schwartz-Cornil, Vanessa Contreras, Nicolas Bertho, Hans Nauwynck |
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| Přispěvatelé: | Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Vaccibody AS, Partenaires INRAE, Université de Lausanne (UNIL), Plateforme d'Infectiologie Expérimentale (PFIE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratory of Virology, Faculty of Veterinary Medicine, Universiteit Gent = Ghent University [Belgium] (UGENT), European Union’s Horizon 2020 Programme Grant Agreement number 633184, DIM-ASTREA, Department of Animal Health at INRA (Grant number AST160021), French government 'Programme d’Investissements d’Avenir' (PIA), grant ANR-11-INBS- 0008, Université de Lausanne = University of Lausanne (UNIL), Universiteit Gent = Ghent University (UGENT), Schwartz, Isabelle |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
COMMERCIAL ELISAS Swine Virologie T-Lymphocytes viruses lcsh:QR1-502 Antibodies Viral Epitope lcsh:Microbiology NONSTRUCTURAL PROTEINS 0302 clinical medicine Animals Antibodies Viral/blood Antibody Formation Immunity Cellular Immunization Schedule Organisms Genetically Modified/genetics Organisms Genetically Modified/immunology Porcine Reproductive and Respiratory Syndrome/prevention & control Porcine respiratory and reproductive syndrome virus/genetics Porcine respiratory and reproductive syndrome virus/immunology T-Lymphocytes/immunology Vaccines Attenuated/administration & dosage Vaccines Attenuated/genetics Vaccines Attenuated/immunology Vaccines DNA/administration & dosage Vaccines DNA/genetics Vaccines DNA/immunology Viral Vaccines/administration & dosage Viral Vaccines/genetics Viral Vaccines/immunology DNA vaccine PRRSV antigen-presenting cell targeting modified-live vaccine pigs INFECTION Vaccines DNA Attenuated vaccine IFN-GAMMA biology IMMUNE-RESPONSES 3. Good health Infectious Diseases [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Veterinary medicine and animal Health stratégie vaccinale Antibody réponse immunitaire vaccin à adn ANTIGEN Porcine Reproductive and Respiratory Syndrome Vaccines Attenuated DENDRITIC CELLS Article antigen-presenting cell DNA vaccination 03 medical and health sciences DELIVERY Immune system Antigen Virology Porcine respiratory and reproductive syndrome virus Veterinary Sciences Antigen-presenting cell targeting [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Organisms Genetically Modified Biology and Life Sciences Viral Vaccines Porcine reproductive and respiratory syndrome virus biology.organism_classification CROSS-PROTECTION Médecine vétérinaire et santé animal maladie respiratoire 030104 developmental biology maladie virale biology.protein porc 030215 immunology GENERATION |
| Zdroj: | Viruses Volume 11 Issue 6 VIRUSES-BASEL Viruses, vol. 11, no. 6 Viruses, MDPI, 2019, 11 (6), pp.1-19. ⟨10.3390/v11060551⟩ Viruses, Vol 11, Iss 6, p 551 (2019) Viruses, 2019, 11 (6), pp.1-19. ⟨10.3390/v11060551⟩ Viruses 6 (11), 1-19. (2019) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v11060551 |
| Popis: | The Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) induces reproductive disorders in sows and respiratory illnesses in growing pigs and is considered as one of the main pathogenic agents responsible for economic losses in the porcine industry worldwide. Modified live PRRSV vaccines (MLVs) are very effective vaccine types against homologous strains but they present only partial protection against heterologous viral variants. With the goal to induce broad and cross-protective immunity, we generated DNA vaccines encoding B and T antigens derived from a European subtype 1 strain that include T-cell epitope sequences known to be conserved across strains. These antigens were expressed either in a native form or in the form of vaccibodies targeted to the endocytic receptor XCR1 and CD11c expressed by different types of antigen-presenting cells (APCs). When delivered in skin with cationic nanoparticles and surface electroporation, multiple DNA vaccinations as a stand-alone regimen induced substantial antibody and T-cell responses, which were not promoted by targeting antigens to APCs. Interestingly, a DNA-MLV prime&ndash boost strategy strongly enhanced the antibody response and broadened the T-cell responses over the one induced by MLV or DNA-only. The anti-nucleoprotein antibody response induced by the DNA-MLV prime&ndash boost was clearly promoted by targeting the antigen to CD11c and XCR1, indicating a benefit of APC-targeting on the B-cell response. In conclusion, a DNA-MLV prime&ndash boost strategy, by enhancing the potency and breadth of MLV vaccines, stands as a promising vaccine strategy to improve the control of PRRSV in infected herds. |
| Databáze: | OpenAIRE |
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