Co-expression of S100A14 and S100A16 correlates with a poor prognosis in human breast cancer and promotes cancer cell invasion
| Autor: | Sayaka Yoshida, Keisuke Nishiura, Namiko Shishito, Tomiko Miura, Takashi Sugino, Hideki Chiba, Ayumi Hozumi, Mizuko Tanaka, Naoki Ichikawa-Tomikawa, Tohru Ohtake |
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| Jazyk: | angličtina |
| Předmět: |
Adult
CA15-3 Pathology medicine.medical_specialty Cancer Research Gene Expression Breast Neoplasms Immunofluorescence Metastasis Breast cancer Invasion Cell Movement Surgical oncology Cell Line Tumor Biomarkers Tumor medicine Genetics Humans Actin Aged Neoplasm Staging medicine.diagnostic_test business.industry Calcium-Binding Proteins S100 Proteins Cancer Middle Aged Prognosis medicine.disease Immunohistochemistry Actins S100A16 Protein Transport S100A14 Oncology Tumor progression Gene Knockdown Techniques Cancer cell Cancer research Female Neoplasm Grading business Protein Binding Research Article |
| Zdroj: | BMC Cancer |
| ISSN: | 1471-2407 |
| DOI: | 10.1186/s12885-015-1059-6 |
| Popis: | Background S100 family proteins have recently been identified as biomarkers in various cancers. Of this protein family, S100A14 and S100A16 are also believed to play an important role in tumor progression. The aim of the present study was to clarify the clinical significance and functional role of these molecules in breast cancer. Methods In a clinical study, an immunohistochemical analysis of S100A14 and S100A16 expression in archival specimens of primary tumors of 167 breast cancer patients was performed. The relationship of S100A14 and S100A16 expression to patient survival and clinicopathological variables was statistically analyzed. In an experimental study, the subcellular localization and function of these molecules was examined by using the human breast cancer cell lines MCF7 and SK-BR-3, both of which highly express S100A14 and S100A16 proteins. Cells transfected with expression vectors and siRNA for these genes were characterized using in vitro assays for cancer invasion and metastasis. Results Immunohistochemical analysis of 167 breast cancer cases showed strong cell membrane staining of S100A14 (53% of cases) and S100A16 (31% of cases) with a significant number of cases with co-expression (p |
| Databáze: | OpenAIRE |
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