High-performance liquid chromatography method using ultraviolet detection for the quantification of aripiprazole and dehydroaripiprazole in psychiatric patients
| Autor: | Pedro Dorado, Eva M Peñas-Lledó, María Eugenia G Naranjo, Alfredo de la Rubia, Idilio González, Adrián LLerena, Antonio P. González, Fernando de Andrés |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Analyte Aripiprazole Quinolones High-performance liquid chromatography Piperazines Limit of Detection medicine Humans Pharmacology (medical) In patient Psychiatry Dehydroaripiprazole Chromatography High Pressure Liquid Aged Detection limit Chromatography medicine.diagnostic_test Chemistry Mental Disorders Reproducibility of Results Middle Aged Quantitative determination respiratory tract diseases Therapeutic drug monitoring Female Spectrophotometry Ultraviolet Drug Monitoring Antipsychotic Agents medicine.drug |
| Zdroj: | Drug Metabolism and Drug Interactions. 27 |
| ISSN: | 2191-0162 0792-5077 |
| DOI: | 10.1515/dmdi-2012-0016 |
| Popis: | Background Aripiprazole (ARI) is an antipsychotic drug that is metabolized to dehydroaripiprazole (DARI) by CYP2D6. Because of the large interindividual variability in ARI and DARI plasma concentrations, therapeutic drug monitoring may be of use in psychiatric patients during treatment with ARI. The aim of the present study was to develop a simple and reliable method for the quantitative determination of ARI and DARI in plasma using liquid-liquid extraction and reverse-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The method was tested in psychiatric patients during regular treatment with ARI. Methods Separation was by the liquid-liquid method, and UV detection at 254 nm. Linear responses for ARI and DARI were obtained between 2 and 1000 ng/mL, and precision assays were lower than 10.4 for both analytes. Results Lower limit of quantification and detection were 1 and 0.38 ng/mL for ARI and 0.78 and 0.44 ng/mL for DARI, respectively. The method was successfully applied to plasma samples drawn from 22 patients with concentrations ranging between 2 and 189 ng/mL for ARI and between 11 and 359 ng/mL for DARI. Conclusions The chromatographic method developed has been demonstrated to be sensitive and reliable for the measurement of ARI and DARI simultaneously in human plasma, and the present method represents an alternative procedure to evaluate plasma concentration in patients during treatment with ARI. |
| Databáze: | OpenAIRE |
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