Regulation of Leishmania (L.) amazonensis protein expression by host T cell dependent responses: differential expression of oligopeptidase B, tryparedoxin peroxidase and HSP70 isoforms in amastigotes isolated from BALB/c and BALB/c nude mice

Autor: Marcello André Barcinski, Leonardo Garcia Velasquez, José Matheus Camargo Bonatto, Priscila Camillo Teixeira, Edecio Cunha-Neto, Beatriz S. Stolf, Ana Paula Lepique, Eloiza de Rezende
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: PLoS Neglected Tropical Diseases, Vol 9, Iss 2, p e0003411 (2015)
PLoS Neglected Tropical Diseases
ISSN: 1935-2735
1935-2727
Popis: Leishmaniasis is an important disease that affects 12 million people in 88 countries, with 2 million new cases every year. Leishmania amazonensis is an important agent in Brazil, leading to clinical forms varying from localized (LCL) to diffuse cutaneous leishmaniasis (DCL). One interesting issue rarely analyzed is how host immune response affects Leishmania phenotype and virulence. Aiming to study the effect of host immune system on Leishmania proteins we compared proteomes of amastigotes isolated from BALB/c and BALB/c nude mice. The athymic nude mice may resemble patients with diffuse cutaneous leishmaniasis, considered T-cell hyposensitive or anergic to Leishmania´s antigens. This work is the first to compare modifications in amastigotes’ proteomes driven by host immune response. Among the 44 differentially expressed spots, there were proteins related to oxidative/nitrosative stress and proteases. Some correspond to known Leishmania virulence factors such as OPB and tryparedoxin peroxidase. Specific isoforms of these two proteins were increased in parasites from nude mice, suggesting that T cells probably restrain their posttranslational modifications in BALB/c mice. On the other hand, an isoform of HSP70 was increased in amastigotes from BALB/c mice. We believe our study may allow identification of potential virulence factors and ways of regulating their expression.
Author Summary Leishmaniasis is an important disease that affects 12 million people in 88 countries. Leishmania amazonensis is an important agent of leishmaniasis in Brazil, leading mainly to localized (LCL) and sometimes to diffuse cutaneous leishmaniasis (DCL), depending on the host immune response to infection. We believe that host immune response affects not only the clinical form and survival of Leishmania, but also the phenotype and virulence of the parasite. To analyze the effects of the host on Leishmania phenotype, we compared protein expression (proteome) of parasites isolated from wild type mice and from mice lacking T cells. We identified some protein isoforms differentially expressed, which may further be studied as potential virulence factors.
Databáze: OpenAIRE
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