Effect of Hyperhomocystinemia and Hypertension on Endothelial Function in Methylenetetrahydrofolate Reductase–Deficient Mice
| Autor: | Mario Fritsch Neves, Marc Iglarz, Farhad Amiri, Agostino Virdis, Rhian M. Touyz, E L Schiffrin, Rima Rozen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Xanthine Oxidase
medicine.medical_specialty Hyperhomocysteinemia Endothelium Biological Availability In Vitro Techniques Nitric Oxide Nitric oxide Mice chemistry.chemical_compound Superoxides Internal medicine medicine Animals Endothelial dysfunction Aorta Methylenetetrahydrofolate Reductase (NADPH2) Mice Knockout biology Superoxide Angiotensin II NADPH Oxidases Tetrahydrobiopterin medicine.disease Acetylcholine digestive system diseases Mesenteric Arteries Vasodilation Nitric oxide synthase NG-Nitroarginine Methyl Ester Endocrinology medicine.anatomical_structure chemistry Methylenetetrahydrofolate reductase Hypertension biology.protein Endothelium Vascular Nitric Oxide Synthase Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 23:1352-1357 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000083297.47245.da |
| Popis: | Objective— We evaluated the effect of hyperhomocystinemia and angiotensin (Ang) II on vascular function and structure in methylenetetrahydrofolate reductase knockout mice ( Mthfr +/− ). Methods and Results— Mthfr +/− and controls ( Mthfr +/+ ) received Ang II (400 ng/kg per min SC) or saline (14 days). Blood pressure, similar in Mthfr +/− and Mthfr +/+ , was increased by Ang II. Acetylcholine- and bradykinin-induced relaxations were impaired in mesenteric resistance arteries (pressurized myograph) in Mthfr +/− and in Ang II–infused Mthfr +/+ mice and additionally blunted in Ang II–infused Mthfr +/− mice. The inhibition by L-NAME on acetylcholine was reduced in Mthfr +/− and in Ang II– Mthfr +/+ and absent in Ang II– Mthfr +/− mice. In these groups, vitamin C improved the response to acetylcholine and restored the inhibition by L-NAME. The media to lumen ratio of small arteries, similar in Mthfr +/− and Mthfr +/+ , was increased by Ang II. Vascular NADPH oxidase activity, similar in Mthfr +/− and Mthfr +/+ , increased after Ang II infusion. Vascular xanthine oxidase activity was also similar in Mthfr +/− and Mthfr +/+ . Superoxide production in the aorta was reduced by sepiapterin and by L-NAME, suggesting that reduced bioavailability of tetrahydrobiopterin and uncoupling of nitric oxide synthase were the origin of increased reactive oxygen species in this model. Conclusions— Mthfr +/− mice show endothelial dysfunction of mesenteric vessels probably attributable to a reduced nitric oxide bioavailability caused by oxidative excess due to uncoupling of nitric oxide synthase without vascular structural alterations. Concurrent Ang II–induced hypertension additionally reduced nitric oxide, increased NADPH oxidase activity, and induced structural alterations. Our findings suggest additive adverse effect of Ang II–dependent hypertension and hyperhomocystinemia on endothelial function. |
| Databáze: | OpenAIRE |
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