Peptidomic analysis of the skin secretions of the frog Pachymedusa dacnicolor
Autor: | Victoria Pando, Erika P. Meneses, Lorena Hernández-Orihuela, Cesar Vicente Ferreira Batista, Rubén Castro-Franco, Oscar Villa-Hernández, Manuel B. Aguilar |
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Rok vydání: | 2010 |
Předmět: |
medicine.medical_treatment
Clinical Biochemistry Antimicrobial peptides Molecular Sequence Data Peptide medicine.disease_cause Biochemistry Peptide Mapping Mass Spectrometry Microbiology medicine Animals Amino Acid Sequence Escherichia coli Peptide sequence Skin chemistry.chemical_classification Protease biology Bacteria Organic Chemistry biology.organism_classification Anti-Bacterial Agents Molecular Weight chemistry Salmonella enterica Anura Frog Skin Sequence Alignment |
Zdroj: | Amino acids. 40(1) |
ISSN: | 1438-2199 |
Popis: | High-resolution mass spectrometry-based peptidomics has been used to characterize several components in electro-stimulated skin secretions of the endemic Mexican frog Pachymedusa dacnicolor. Peptide mass screening performed in an Orbitrap-XL mass spectrometer showed that P. dacnicolor skin secretions possess 194 different components with molecular masses ranging mainly from 500 to 6,000 Da. Dozens of molecules were partially sequenced including two novel protease inhibitors. Additionally, one posttranslationally modified bradykinin and two novel dermaseptin-like antimicrobial peptides were fully sequenced. The novel peptide named here DMS-DA5 was fully characterized and showed potent antibacterial activity against various bacteria such as Escherichia coli, Bacillus subtilis, Salmonella enterica serovar typhimurium, and Pseudomonas aeruginosa with minimal inhibitory concentrations from 3.10 to 25.0 microM. |
Databáze: | OpenAIRE |
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