Modifications of band 3 and oxidation level of membrane proteins in senescent erythrocytes
| Autor: | Carlos Cotorruelo, Silvia García Borrás, Nicolás Mufarrege, Liliana Racca, María Alejandra Ensinck, Amelia Racca, Claudia Biondi, Angel Rucci |
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| Rok vydání: | 2010 |
| Předmět: |
Erythrocytes
biology Clinical Biochemistry Immunoblotting Membrane Proteins General Medicine Erythrocyte Aging Protein oxidation Blood cell Red blood cell medicine.anatomical_structure Biochemistry Membrane protein Ageing Anion Exchange Protein 1 Erythrocyte biology.protein medicine Humans Electrophoresis Polyacrylamide Gel Band 3 Polyacrylamide gel electrophoresis Percoll Oxidation-Reduction Protein Processing Post-Translational |
| Zdroj: | Clinical biochemistry. 43(13-14) |
| ISSN: | 1873-2933 |
| Popis: | Objectives To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). Design and methods SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups. Results Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC. Conclusions These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan. |
| Databáze: | OpenAIRE |
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