Three-dimensional super-resolution fluorescence imaging of DNA
| Autor: | Sevim Yardimci, Samantha Y.A. Terry, Hasan Yardimci, Daniel R. Burnham |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Materials science lcsh:Medicine 02 engineering and technology Astigmatism 010402 general chemistry 01 natural sciences Chromosomes Article 03 medical and health sciences chemistry.chemical_compound Jurkat Cells Optical imaging Imaging Three-Dimensional Single-molecule biophysics Microscopy medicine Molecule Humans lcsh:Science Metaphase 030304 developmental biology 0303 health sciences Multidisciplinary lcsh:R Optical Imaging DNA medicine.disease 021001 nanoscience & nanotechnology Superresolution Fluorescence 0104 chemical sciences Kinetics 030104 developmental biology chemistry Microscopy Fluorescence Biophysics lcsh:Q 0210 nano-technology Protein Binding |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes, unravelling their structural features. |
| Databáze: | OpenAIRE |
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