High prevalence of Chlamydia pneumoniae DNA in peripheral blood mononuclear cells in patients with cardiovascular disease and in middle-aged blood donors
| Autor: | Lisbeth Slunga Birgander, Göran Wadell, Erik Jidell, Annika Allard, Urban Kumlin, Anders Waldenström, Per Juto, Jens Boman, Jenny Forsberg, Stefan Söderberg, Kenneth Persson |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
DNA Bacterial Male Blood Donors Coronary Disease medicine.disease_cause Coronary Angiography Peripheral blood mononuclear cell law.invention Serology law Prevalence Immunology and Allergy Medicine Humans Polymerase chain reaction Aged Aged 80 and over Bartonella henselae Chlamydia biology business.industry Chlamydia Infections Chlamydophila pneumoniae Middle Aged biology.organism_classification medicine.disease Antibodies Bacterial Infectious Diseases Immunology biology.protein Leukocytes Mononuclear Female Antibody business Nested polymerase chain reaction |
| Zdroj: | The Journal of infectious diseases. 178(1) |
| ISSN: | 0022-1899 |
| Popis: | Nested polymerase chain reaction (nPCR) demonstrated the presence of Chlamydia pneumoniae-specific DNA in peripheral blood mononuclear cells (PBMC). PBMC samples were obtained from 103 consecutive patients (62 male, 41 female) aged 22-85 years (mean, 64) admitted for coronary angiography because of suspected coronary heart disease and from 52 blood donors (43 male, 9 female) aged 40-64 years (mean, 49). Of the 101 evaluable patients, 60 (59%) were identified by nPCR assay as C. pneumoniae DNA carriers; C. pneumoniae-specific microimmunofluorescence (MIF) serology confirmed exposure to the bacterium in 57 (95%) of the 60 nPCR-positive patients. Among the 52 blood donors, the nPCR assay identified 24 (46%) C. pneumoniae DNA carriers, all of whom were positive by C. pneumoniae-specific serology. Thirty-two patients (32%) and 23 blood donors (44%) were MIF antibody-positive but repeatedly nPCR-negative; Bartonella henselae- or Bartonella quintana-specific antibodies were not detected among any of these subjects. In this study, C. pneumoniae DNA was common in PBMC of patients with coronary heart disease and in middle-aged blood donors. |
| Databáze: | OpenAIRE |
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