Enzyme-Linked Immunosorbent Assay of Mycotoxins Using Nylon Bead and Terasaki Plate Solid Phases
| Autor: | Fun S. Chu, J. J. Pestka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Detection limit Aflatoxin Chromatography Chemistry Toxin technology industry and agriculture food and beverages Bead medicine.disease_cause Microbiology chemistry.chemical_compound Microtiter plate Enzyme visual_art medicine visual_art.visual_art_medium Solid phases Mycotoxin Food Science |
| Zdroj: | Journal of food protection. 47(4) |
| ISSN: | 1944-9097 |
| Popis: | Nylon beads and Terasaki plates were tested as solid phases for the enzyme-linked immunosorbent assay (ELISA) of the mycotoxins aflatoxin B1 (AFB1), aflatoxin M1 (AFM1) and T-2 toxin. Both methods had detection limits comparable to that of mycotoxin microtiter plate ELISAs. Using the nylon bead ELISA, ELISA competition curves for AFB1, AFM1 and T-2 toxin exhibited linear response between 1.0 to 100, 0.1 to 100, and 0.1 to 10.0 ng/ml, respectively. Response ranges for Terasaki plate ELISAs of AFB1, AFM1 and T-2 toxin were 1.0 to 50, 0.05 to 0.50, and 0.5 to 1.0 ng/ml, respectively. The new procedures did not require specialized instrumentation and may be used as an economical screening method for mycotoxins in the field and to diagnose certain mycotoxicoses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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