Distribution and activity of anaerobic ammonium-oxidising bacteria in natural freshwater wetland soils
| Autor: | Hong-sheng Wu, Xu Liu, Hai-xiang Cheng, Li-dong Shen, Zhiqiu Gao, Qian-Qi Ren, Ji Li |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
030106 microbiology Wetland Fresh Water Biology Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Soil RNA Ribosomal 16S Botany Ammonium Compounds Ammonium Anaerobiosis Nitrogen cycle Phylogeny Soil Microbiology geography geography.geographical_feature_category Bacteria Stable isotope ratio General Medicine Nitrogen Cycle 16S ribosomal RNA biology.organism_classification chemistry Anammox Genes Bacterial Isotope Labeling Wetlands Anaerobic exercise Oxidation-Reduction Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 100(7) |
| ISSN: | 1432-0614 |
| Popis: | Anaerobic ammonium oxidation (anammox) process plays a significant role in the marine nitrogen cycle. However, the quantitative importance of this process in nitrogen removal in wetland systems, particularly in natural freshwater wetlands, is still not determined. In the present study, we provided the evidence of the distribution and activity of anammox bacteria in a natural freshwater wetland, located in southeastern China, by using (15)N stable isotope measurements, quantitative PCR assays and 16S rRNA gene clone library analysis. The potential anammox rates measured in this wetland system ranged between 2.5 and 25.5 nmol N2 g(-1) soil day(-1), and up to 20% soil dinitrogen gas production could be attributed to the anammox process. Phylogenetic analysis of 16S rRNA genes showed that anammox bacteria related to Candidatus Brocadia, Candidatus Kuenenia, Candidatus Anammoxoglobus and two novel anammox clusters coexisted in the collected soil cores, with Candidatus Brocadia and Candidatus Kuenenia being the dominant anammox genera. Quantitative PCR of hydrazine synthase genes showed that the abundance of anammox bacteria varied from 2.3 × 10(5) to 2.2 × 10(6) copies g(-1) soil in the examined soil cores. Correlation analyses suggested that the soil ammonium concentration had significant influence on the activity of anammox bacteria. On the basis of (15)N tracing technology, it is estimated that a total loss of 31.1 g N m(-2) per year could be linked the anammox process in the examined wetland. |
| Databáze: | OpenAIRE |
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