Glomerular Morphology and Renal Function after Warm Ischemia by Main Artery or Selective Clamping in a Porcine Model
| Autor: | Leonardo de Albuquerque dos Santos Abreu, Gustavo R. Bechara, Marco A. Pereira-Sampaio, José Aurelino Damasceno-Ferreira, Waldemar S. Costa, Francisco J.B. Sampaio, Diogo Benchimol de Souza |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Urology Kidney Glomerulus Sus scrofa 030232 urology & nephrology Renal function Kidney Constriction Renal Circulation 03 medical and health sciences 0302 clinical medicine Renal Artery Internal medicine medicine.artery Medicine Animals Warm Ischemia Renal artery Renal circulation urogenital system business.industry Anatomy Organ Size Arterial occlusion Clamping medicine.anatomical_structure 030220 oncology & carcinogenesis Models Animal Cardiology business Artery |
| Zdroj: | Urologia internationalis. 99(3) |
| ISSN: | 1423-0399 |
| Popis: | Objective: The study aimed to evaluate the ischemic and non-ischemic areas after selective arterial occlusion by using stereological analysis of glomeruli, and to compare them with main arterial clamping and sham-operated animals. Materials and Methods: Twenty-four male pigs were used in the study. The animals were divided into 3 groups with 8 animals in each as follows: group sham, submitted to laparoscopic dissection of the renal pedicle but not submitted to ischemia; group arterial (A), submitted to left renal artery clamping; and group selective (S), submitted to left renal artery caudal branch occlusion. Groups A and S underwent 30 min of warm ischemia. Left and right kidneys were collected after 21 days and renal fragments were processed for stereological evaluation. Glomerular volume density (Vv[glom]), mean glomerular volume (MGV), and glomerular density were measured. Serum creatinine and urea were assessed preoperatively, 10 days after surgery, and before euthanasia. Results: There was no significant difference among groups with regard to renal function. Renal weight and volume were similar among groups. Also, no difference was observed between the groups with regard to Vv[glom], MGV, and glomerular density, both when compared to its right control or when left kidneys were compared. Conclusions: Selective arterial clamping technique was neither superior nor inferior to main artery clamping. |
| Databáze: | OpenAIRE |
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