Specific detection of all members of the Venezuelan Equine Encephalitis complex: Development of a RT-Nested PCR
| Autor: | Antonio Tenorio, Adrián Alejandro Farías, María Belén Pisano, María Paz Sánchez Seco, Viviana Ré, Marta S. Contigiani |
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| Rok vydání: | 2012 |
| Předmět: |
Time Factors
animal structures Specific detection viruses Alphavirus Polymerase Chain Reaction Sensitivity and Specificity Arbovirus law.invention Encephalitis Virus Venezuelan Equine law Virology medicine Animals Humans Equine Encephalitis Polymerase chain reaction DNA Primers biology Reverse Transcriptase Polymerase Chain Reaction virus diseases biology.organism_classification medicine.disease Reverse transcriptase Togaviridae Nested polymerase chain reaction |
| Zdroj: | Journal of Virological Methods. 186:203-206 |
| ISSN: | 0166-0934 |
| Popis: | Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance. |
| Databáze: | OpenAIRE |
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