Effects of different radiation doses on the microhardness, superficial morphology, and mineral components of human enamel
| Autor: | Sandra Ribeiro Cunha, Eduardo Rodrigues Fregnani, Pedro Augusto Minorin Mendes Ramos, Cecília Maria Kalil Haddad, Felipe Paiva Fonseca, Ana Cecília Correa Aranha |
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| Rok vydání: | 2017 |
| Předmět: |
Molar
Materials science Morphology (linguistics) Surface Properties Scanning electron microscope Dentistry In Vitro Techniques Radiation Dosage Indentation hardness 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Hardness Tests Dental Enamel General Dentistry Acrylic resin Enamel paint business.industry Radiation dose Spectrometry X-Ray Emission 030206 dentistry Cell Biology General Medicine Otorhinolaryngology chemistry 030220 oncology & carcinogenesis visual_art Microscopy Electron Scanning visual_art.visual_art_medium Glutaraldehyde business |
| Zdroj: | Archives of Oral Biology. 80:130-135 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2017.04.007 |
| Popis: | Objective To evaluate the effects of three different radiotherapy doses (20, 40, and 70 Gy) on the microhardness, superficial morphology, and mineral content (based on Ca and P values) of three different depths of human enamel (cervical, middle, and occlusal). Design Thirty-four third molars were cut, separated, and prepared. Microhardness samples (n = 30) were embedded in acrylic resin and then polished, and depths were delimited. Microhardness tests were performed on cervical, middle, and occlusal enamel pre- and post-radiotherapy with a load of 50 g for 30 s. For the scanning electron microscopy (SEM) analysis (n = 4) and energy dispersive X-ray spectroscopy (EDS) (n = 12), samples were fixed in a 3% glutaraldehyde solution, washed in 0.1 M cacodylate solution, and dehydrated in crescent concentrations of ethanol. Microhardness data were tested for significant differences using a two-way analysis of variance (ANOVA) and Tukey’s test (p Results The results showed a decrease in microhardness values only in the cervical enamel, regardless of the radiation dose used; no morphological or mineral change was observed. Conclusion Radiotherapy can affect the microhardness values of only cervical enamel without compromising the morphological or mineral (Ca and P) content at any depth. |
| Databáze: | OpenAIRE |
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