DNA damage and oxidative stress induced by imidacloprid exposure in different tissues of the Neotropical fish Prochilodus lineatus

Autor: Cláudia B.R. Martinez, Raphael D’Anna Acayaba, Carlos Eduardo Delfino Vieira, Cassiana Carolina Montagner Raimundo, María Rita Pérez
Rok vydání: 2017
Předmět:
Gill
Gills
Insecticides
Environmental Engineering
Erythrocytes
DNA damage
Health
Toxicology and Mutagenesis
0211 other engineering and technologies
Physiology
Fresh Water
02 engineering and technology
010501 environmental sciences
medicine.disease_cause
Kidney
01 natural sciences
chemistry.chemical_compound
Neonicotinoids
Imidacloprid
medicine
Environmental Chemistry
Animals
Tissue Distribution
Biotransformation
0105 earth and related environmental sciences
021110 strategic
defence & security studies
biology
Public Health
Environmental and Occupational Health
General Medicine
General Chemistry
biology.organism_classification
Nitro Compounds
Pollution
Acute toxicity
Oxidative Stress
chemistry
Liver
Neotropical fish
Freshwater fish
Characiformes
Genotoxicity
Oxidative stress
Biomarkers
Water Pollutants
Chemical
DNA Damage
Zdroj: Chemosphere. 195
ISSN: 1879-1298
Popis: Imidacloprid (IMI), a systemic neonicotinoid insecticide widely used in worldwide scale, is reported in freshwater bodies. Nevertheless, there is a lack of information about IMI sublethal effects on freshwater fish. Thus, the aim of this study was to identify the potential hazard of this insecticide to the South American fish Prochilodus lineatus exposed for 120 h to four IMI concentrations (1.25, 12.5, 125, and 1250 μg L−1). A set of biochemical, genotoxic and physiological biomarkers were evaluated in different organs of the fish. IMI exposure induced significant changes in the enzymatic profiles of P. lineatus, with alterations in the activity of biotransformation and antioxidant enzymes in different tissues. Redox balance of the tissues was affected, since oxidative damage such as lipoperoxidation (LPO) and protein carbonylation (PCC) were evidenced in the liver, gills, kidney and brain of fish exposed to different IMI concentrations. Fish exposed to all IMI concentrations showed decreased blood glucose indicating an increase of energetic demand. DNA damage was evidenced by the comet test, in the erythrocytes of fish all the concentrations evaluated. We integrated these results in the Integrated Biomarker Response (IBR) index, which evidenced that the organs most affected by IMI exposure were the liver and kidney, followed by the gills. Our results highlight the importance of investigating different target tissues after IMI exposure and show the sublethal effects of IMI in some of them; they also warn to the possible consequences that fish living in freshwater ecosystems can suffer due to IMI exposure.
Databáze: OpenAIRE
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