Immunohistochemistry, histopathology and infrared spectral histopathology of colon cancer tissue sections
| Autor: | Klaus Gerwert, Andrea Tannapfel, Angela Kallenbach-Thieltges, Axel Mosig, Frederik Großerüschkamp, Max Diem |
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| Rok vydání: | 2012 |
| Předmět: |
Diagnostic Imaging
medicine.medical_specialty Pathology Spectrophotometry Infrared Colorectal cancer Colon General Physics and Astronomy Biology General Biochemistry Genetics and Molecular Biology Automation Spectroscopy Fourier Transform Infrared medicine Image Processing Computer-Assisted Cluster Analysis Humans Scattering Radiation General Materials Science Models Statistical General Engineering Reproducibility of Results General Chemistry medicine.disease Immunohistochemistry Tissue sections Colonic Neoplasms Mutation Colon tissue Histopathology Colon adenocarcinoma Monte Carlo Method Algorithms |
| Zdroj: | Journal of biophotonics. 6(1) |
| ISSN: | 1864-0648 |
| Popis: | During the past years, many studies have shown that infrared spectral histopathology (SHP) can distinguish different tissue types and disease types independently of morphological criteria. In this manuscript, we report a comparison of immunohistochemical (IHC), histopathological and spectral histopathological results for colon cancer tissue sections. A supervised algorithm, based on the "random forest" methodology, was trained using classical histopathology, and used to automatically identify colon tissue types, and areas of colon adenocarcinoma. The SHP images subsequently were compared to IHC-based images. This comparison revealed excellent agreement between the methods, and demonstrated that label-free SHP detects compositional changes in tissue that are the basis of the sensitivity of IHC. |
| Databáze: | OpenAIRE |
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