Sputum microRNA‐screening reveals Prostaglandin EP3 receptor as selective target in allergen‐specific immunotherapy
| Autor: | Antonia Kloss, Susanne Krauss-Etschmann, Adam Chaker, Ulrich M. Zissler, Julia T. Ullmann, Anna Erb, Madlen Oelsner, Julia Esser-von Bieren, Alexandra Kurz, Carsten B. Schmidt-Weber, Ferdinand Guerth, Constanze A. Jakwerth, Lynn S Zur Bonsen, Marlene Winkler, Mirjam Plaschke, Lisa Pechtold, Josephine Kau |
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| Rok vydání: | 2020 |
| Předmět: |
Microarray
medicine.medical_treatment Receptors Prostaglandin Immunology Prostaglandin Context (language use) chemistry.chemical_compound medicine Humans Immunology and Allergy Lymphocytes Prostaglandin E2 Asthma Microarray analysis techniques business.industry Sputum Immunotherapy Allergens medicine.disease Rhinitis Allergic Immunity Innate ddc MicroRNAs Allergen-specific Immunotherapy Allergic Asthma/rhinitis Induced Sputum Microrna chemistry Desensitization Immunologic Prostaglandins medicine.symptom business medicine.drug |
| Zdroj: | Clin. Exp. Allergy, DOI: 10.1111/cea.14013 (2021) |
| DOI: | 10.1111/cea.14013 |
| Popis: | BACKGROUND Several microRNAs (miRs) have been described as potential biomarkers in liquid biopsies and in the context of allergic asthma, while therapeutic effects on the airway expression of miRs remain elusive. In this study, we investigated epigenetic miR-associated mechanisms in the sputum of grass pollen-allergic patients with and without allergen-specific immunotherapy (AIT). METHODS Induced sputum samples of healthy controls (HC), AIT-treated and -untreated grass pollen-allergic rhinitis patients with (AA) and without asthma (AR) were profiled using miR microarray and whole-transcriptome microarray analysis of the same samples. miR targets were predicted in silico and used to identify inverse regulation. Local PGE2 levels were measured using ELISA. RESULTS Two hundred and fifty nine miRs were upregulated in the sputum of AA patients compared with HC, while only one was downregulated. The inverse picture was observed in induced sputum of AIT-treated patients: while 21 miRs were downregulated, only 4 miRs were upregulated in asthmatics upon AIT. Of these 4 miRs, miR-3935 stood out, as its predicted target PTGER3, the prostaglandin EP3 receptor, was downregulated in treated AA patients compared with untreated. The levels of its ligand PGE2 in the sputum supernatants of these samples were increased in allergic patients, especially asthmatics, and downregulated after AIT. Finally, local PGE2 levels correlated with ILC2 frequencies, secreted sputum IL-13 levels, inflammatory cell load, sputum eosinophils and symptom burden. CONCLUSIONS While profiling the sputum of allergic patients for novel miR expression patterns, we uncovered an association between miR-3935 and its predicted target gene, the prostaglandin E3 receptor, which might mediate AIT effects through suppression of the PGE2 -PTGER3 axis. |
| Databáze: | OpenAIRE |
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