Phosphatidylcholine as a metabolic cue for determining B cell fate and function
| Autor: | Joseph W. Brewer, Viktoriya Solodushko, Robert A. Barrington, Ileana V. Aragon |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
T-Lymphocytes Cellular differentiation Immunology Lymphocyte Activation Article Immunoglobulin G Mice 03 medical and health sciences 0302 clinical medicine Immune system Antigen medicine Animals Choline-Phosphate Cytidylyltransferase Cells Cultured B cell Mice Knockout B-Lymphocytes biology Germinal center Cell Differentiation Germinal Center Molecular biology Immunity Humoral 030104 developmental biology medicine.anatomical_structure Immunoglobulin class switching 030220 oncology & carcinogenesis Phosphatidylcholines Unfolded Protein Response biology.protein Antibody Immunologic Memory |
| Zdroj: | Cellular Immunology. 310:78-88 |
| ISSN: | 0008-8749 |
| Popis: | In activated B cells, increased production of phosphatidylcholine (PtdCho), the most abundant cellular phospholipid, is handled primarily by the CDP-choline pathway. B cell-specific deletion of CTP:phosphocholine cytidylyltransferase α (CCTα), the rate-limiting enzyme in the CDP-choline pathway, led to augmented IgM secretion and reduced IgG production, suggesting that PtdCho synthesis is required for germinal center reactions. To specifically assess whether PtdCho influences B cell fate during germinal center responses, we examined immune responses in mice whereby PtdCho synthesis is disrupted in B cells that have undergone class switch recombination to IgG1 (referred to as either Cγ1wt/wt, Cγ1Cre/wt or Cγ1Cre/Cre based on Cre copy number). Serum IgG1 was markedly reduced in naive Cγ1Cre/wt and Cγ1Cre/Cre mice, while levels of IgM and other IgG subclasses were similar between Cγ1Cre/wt and Cγ1wt/wt control mice. Serum IgG2b titers were notably reduced and IgG3 titers were increased in Cγ1Cre/Cre mice compared with controls. Following immunization with T cell-dependent antigen NP-KLH, control mice generated high titer IgG anti-NP while IgG anti-NP titers were markedly reduced in both immunized Cγ1Cre/wt and Cγ1Cre/Cre mice. Correspondingly, the frequency of NP-specific IgG antibody-secreting cells was also reduced in spleens and bone marrow of Cγ1Cre/wt and Cγ. 1Cre/Cre mice compared to control mice. Interestingly, though antigen-specific IgM B cells were comparable between Cγ1Cre/wt, Cγ1Cre/Cre and control mice, the frequency and number of IgG1 NP-specific B cells was reduced only in Cγ1Cre/Cre mice. These data indicate that PtdCho is required for the generation of both germinal center-derived B cells and antibody-secreting cells. Further, the reduction in class-switched ASC but not B cells in Cγ1Cre/wt mice suggests that ASC have a greater demand for PtdCho compared to germinal center B cells. |
| Databáze: | OpenAIRE |
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