Human herpesvirus 6 (HHV-6)-associated dysfunction of blood monocytes
| Autor: | Donald R. Carrigan, Eileen M. Burd |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
Herpesvirus 6 Human viruses medicine.medical_treatment Monocytes Virus chemistry.chemical_compound Virology medicine Humans Opsonin Protein kinase C Respiratory Burst biology Monocyte Growth factor Zymosan Herpesviridae Infections biology.organism_classification Molecular biology Respiratory burst Infectious Diseases medicine.anatomical_structure chemistry Luminescent Measurements Immunology Tetradecanoylphorbol Acetate Human herpesvirus 6 |
| Zdroj: | Virus Research. 29:79-90 |
| ISSN: | 0168-1702 |
| Popis: | HHV-6 is a recently described member of the herpesvirus family. HHV-6-associated marrow failure and interstitial pneumonitis where macrophages are the primary infected cell type have been described in marrow transplant patients (Carrigan, 1991; Drobyski et al., 1993). In recent studies we have shown that exposure of normal human marrow to HHV-6 GS (a type A strain) or several type B strains resulted in suppression of growth factor induced outgrowth of macrophages by > 90% (Burd and Carrigan, 1993). Additional experiments using HHV-6 GS to characterize the effects of the virus on peripheral blood monocytes showed that the respiratory burst capacity of these cells as determined by luminol-enhanced chemiluminescence using phorbol myristate acetate as a trigger was decreased by 83% ± 13% in a series of 5 experiments. The decreased respiratory burst was evident as early as 15 min after exposure to virus. Experiments in which cells were separated on a fluorescence activated cell sorter prior to respiratory burst assay showed that the response was mediated solely by peripheral blood monocytes. The respiratory burst response of virus-exposed cells to opsonized zymosan was not affected, indicating that the virus may selectively interfere with the protein kinase C pathway of cellular activation. Ultracentrifugation of stock material to remove infectious virus showed that the suppressive factor was associated with the supernatant fraction. These findings suggest that HHV-6 infection may be associated with a defect in one of the major monocyte activation pathways, and this could be of importance with respect to persistent infection by HHV-6 in immune compromised patients. |
| Databáze: | OpenAIRE |
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