Two galactomannan preparations from seeds from Mimosa scabrella (bracatinga): Complexation with oxovanadium(IV/V) and cytotoxicity on HeLa cells
| Autor: | Guilhermina Rodrigues Noleto, Carmen Lúcia de Oliveira Petkowicz, Ana Lucia Ramalho Mercê, Fany Reicher, Maria Benigna M. Oliveira, Miguel D. Noseda, Stelia Carolina Mendez-Sanchez |
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| Rok vydání: | 2009 |
| Předmět: |
Magnetic Resonance Spectroscopy
Mimosa Cell Survival Stereochemistry Potentiometric titration Polysaccharide Biochemistry Mannans Inorganic Chemistry HeLa Galactomannan chemistry.chemical_compound Spectroscopy Fourier Transform Infrared Humans Mimosa scabrella Cytotoxicity Cell Proliferation chemistry.chemical_classification biology Chemistry Temperature Galactose Hydrogen-Ion Concentration biology.organism_classification NMR spectra database Enzyme Seeds Potentiometry Vanadates HeLa Cells Nuclear chemistry |
| Zdroj: | Journal of Inorganic Biochemistry. 103:749-757 |
| ISSN: | 0162-0134 |
| DOI: | 10.1016/j.jinorgbio.2009.01.018 |
| Popis: | Two galactomannans, GALMAN-A and GALMAN-B, were isolated from seeds of Mimosa scabrella (bracatinga), with deactivation and exposure to native enzymes, respectively. They were treated with oxovanadium(IV) and oxovanadium(V), designated (VO(2+)/VO(3+)) to form GALMAN-A:VO(2+)/VO(3+) and GALMAN-B:VO(2+)/VO(3+) complexes, respectively. The potentiometric studies provided the binding constants for the complexes and the resulting complexed species were a function of pH. (51)V NMR spectra of GALMAN-A:VO(2+)/VO(3+) and GALMAN-B:VO(2+)/VO(3+) at pH 7.8 and at 30 degrees C indicated the occurrence of two types of complexes formed by oxovanadium ions and galactomannans. GALMAN-A:VO(2+)/VO(3+) and GALMAN-B:VO(2+)/VO(3+) caused loss of HeLa cells viability at concentrations of 50-200microg/mL. GALMAN-A:VO(2+)/VO(3+) exhibited low toxicity for 24h, although GALMAN-B:VO(2+)/VO(3+) was extremely toxic, since 50microg/mL was sufficient to decrease HeLa cell viability after 48h by 60%. GALMAN-A gave rise to a slight increase in cell proliferation after 48h at 100microg/mL, whereas GALMAN-B promoted a slight decrease at concentrations of 50-100microg/mL. GALMAN-A:VO(2+)/VO(3+) and GALMAN-B:VO(2+)/VO(3+) exhibited a significant decrease in cell proliferation after 48h, each reaching 60% inhibition at 5-10microg/mL. The complexes which caused this effect were at concentrations 10 times lower than the uncomplexed polymers. |
| Databáze: | OpenAIRE |
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