Analysis of T cell receptor-gene rearrangement in T cells from the cerebrospinal fluid of patients with multiple sclerosis
| Autor: | H. K. Van Walbeek, J. J. M. Van Dongen, I. Kokkelink, C.J. Lucas, Chris H. Polman, Francien T. M. Rotteveel |
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| Rok vydání: | 1987 |
| Předmět: |
Adult
Male Multiple Sclerosis T-Lymphocytes T cell Immunology Receptors Antigen T-Cell Biology Lymphocyte Activation Antigen medicine Humans Immunology and Allergy Receptor Southern blot T-cell receptor Nucleic Acid Hybridization Gene rearrangement T lymphocyte Middle Aged Molecular biology Clone Cells medicine.anatomical_structure Genes Neurology T-Cell Receptor Gene Immunologic Techniques Electrophoresis Polyacrylamide Gel Neurology (clinical) |
| Zdroj: | Journal of Neuroimmunology. 15:243-249 |
| ISSN: | 0165-5728 |
| Popis: | Characterization of T cells present in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) may contribute to an understanding of the immunopathologic role of these cells. To analyze the T cells in the CSF of MS patients, 30 cloned T cell lines from each of two MS patients were surveyed for their patterns of T cell receptor (TcR) beta-chain gene rearrangement. DNA from the (CSF-derived) T cell clones was digested with a number of restriction endonucleases and the gene rearrangement patterns were analyzed with a T cell receptor beta-chain probe. Southern blot analysis of the DNA of these T cell clones indicated that all had rearrangements of the TcR beta-chain genes, but none of the rearrangements were identical. These results suggest that, if a few clones of specific T cells are involved, they must form a tiny minority in comparison to the total number of T cells in the CSF of MS patients. |
| Databáze: | OpenAIRE |
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