Genome-Wide Profiling of miRNA and mRNA Expression in Alzheimer's Disease
| Autor: | Yong-Hong Wang, Xiao-Tun Zhu, Wan-Sheng Chang, Chuan-Jie Wu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene regulatory network Down-Regulation Computational biology Biology Genome 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Lab/In Vitro Research microRNA Gene silencing Humans Gene Regulatory Networks RNA Messenger KEGG Regulation of gene expression Genome Human Gene Expression Profiling Reproducibility of Results Molecular Sequence Annotation General Medicine CDK5R1 Up-Regulation Gene expression profiling MicroRNAs 030104 developmental biology Huntington Disease Gene Expression Regulation Biological Markers 030217 neurology & neurosurgery |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND Our study aimed to identify key differentially expressed genes (DEGs) and miRNAs (DEmiRNAs) which can serve as potential biomarkers for diagnosis and therapy of Alzheimer's disease (AD). MATERIAL AND METHODS We performed miRNA and mRNA integrated analysis (MMIA) to identify DEGs and DEmiRNAs of AD. The AD-specific DEmiRNAs-targets interaction network was contrasted. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed. Q-RT-PCR was used to verify the expression of selected DEGs and DEmiRNAs. RESULTS We conducted MMIA of AD based on 1 miRNA dataset and 3 mRNA datasets derived from the Gene Expression Omnibus (GEO) database; 1759 DEGs and 12 DEmiRNAs were obtained. DEGs of AD were significantly enriched in Huntington's disease and AD. LRP1, CDK5R1, PLCb2, NDUFA4, and DLG4 were 5 DEGs regulated by 4 DEmiRNAs, including miR-26b-5p, miR-26a-5p, miR-107, and miR-103a-3p. These 4 miRNAs were the top 4 miRNAs covering most DEGs. According to the qRT-PCR results, the expression of PLCβ2, NDUFA4, DLG4, miR-107, and miR-103a-3p was consistent with our integrated analysis. CONCLUSIONS We concluded that LRP1, CDK5R1, PLCβ2, NDUFA4, and DLG4 may play a role in AD regulated by miR-26b-5p, miR-26a-5p, miR-107, and miR-103a-3p. Our findings will contribute to identification of biomarkers and new strategies for drug design for AD treatment. |
| Databáze: | OpenAIRE |
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