Metabolism of dibenzothiophene by a Beijerinckia species
| Autor: | D T Gibson, A L Laborde |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Oxygenase
Magnetic Resonance Spectroscopy food.ingredient Chemical Phenomena Stereochemistry Metabolite Thiophenes Naphthalenes Applied Microbiology and Biotechnology Cell-free system chemistry.chemical_compound food Beijerinckia Cell-Free System Ecology biology Succinates Metabolism biology.organism_classification Pseudomonas putida Chemistry chemistry Biochemistry Dibenzothiophene Oxygenases Pseudomonadaceae Oxidation-Reduction Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 34:783-790 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.34.6.783-790.1977 |
| Popis: | Beijerinckia B8/36 when grown with succinate in the presence of dibenzothiophene, accumulated (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene and dibenzothiophene-5-oxide in the culture medium. Each metabolite was isolated in crystalline form and characterized by a variety of chemical techniques, cis-Naphthalene dihydrodiol dehydrogenase, isolated from Pseudomonas putida, oxidized (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene to a compound that was tentatively identified as 1,2-dihydroxydibenzothiophene. The same product was formed when crude cell extracts of the parent strain of Beijerinckia oxidized (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene under anaerobic conditions. Further metabolism of 1,2-dihydroxydibenzothiophene by heat-treated cell extracts led to the formation of 4[2-(3-hydroxy)-thionaphthenyl]-2-oxo-3-butenoic acid. The latter compound was metabolized by crude cell extracts to 3-hydroxy-2-formylthionaphthene. Further degradation of this metabolite was not observed. |
| Databáze: | OpenAIRE |
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