Transmission Electron Microscopy of Sputum Deposition in the Diagnosis of Pulmonary Alveolar Proteinosis
| Autor: | Hanjing Lv, Huiping Li, Zongmei Zhang, Lan Wang, Haiqing Chu, Xiaojing Li, Benfang Luo, Xia Fang, Xianghua Yi, Xuyou Zhu, Yu Zeng |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Sputum Cytology Pathology medicine.medical_specialty Biopsy Lung biopsy Pulmonary Alveolar Proteinosis Lamellar granule Pathology and Forensic Medicine Microscopy Electron Transmission Structural Biology Humans Medicine Lung Myelin Sheath Aged medicine.diagnostic_test business.industry Sputum Middle Aged respiratory system medicine.disease respiratory tract diseases Pulmonary Alveoli Bronchoalveolar lavage Female Histopathology medicine.symptom business Pulmonary alveolar proteinosis Bronchoalveolar Lavage Fluid |
| Zdroj: | Ultrastructural Pathology. 36:153-159 |
| ISSN: | 1521-0758 0191-3123 |
| DOI: | 10.3109/01913123.2011.639134 |
| Popis: | To clarify the diagnostic value of sputum in pulmonary alveolar proteinosis (PAP) through transmission electron microscopy (TEM) of sputum deposition (SD).Eleven SD samples and 9 bronchoalveolar lavage (BAL) sediments from a PAP group including 11 patients were observed by TEM and compared with sputum direct smear, BAL cytology, and lung biopsy histopathology. Eleven healthy adults were chosen as controls.The 11 sputum smears from the PAP group showed no diagnostic component, but TEM of SD revealed 7 of 11 samples had many myelin-like lamellar bodies with degeneration in the cytoplasm of macrophages, alveolar epithelial cells, and extracellular spaces, which suggested PAP. Especially, 2 patients on whom lung biopsy could not be performed and who failed to be diagnosed by BAL fluid were finally diagnosed by TEM of SD. TEM of BAL sediments showed 7 of 9 cases had diagnostic myelin-like lamellar bodies. No statistical significance was found between BAL fluid and SD by TEM. The control group didn't show diagnostic components by cytology or TEM of SD.TEM of SD is an important noninvasive diagnostic method especially for patients against lung biopsy and BAL. |
| Databáze: | OpenAIRE |
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