Chitosan-based core–shell structured particles for in vivo sustainable gene transfection
| Autor: | Yun Wang, Xuewu Ge, Zhen-xing Gong, Yu-fang Gu, Mozhen Wang, Fu-xing Lin, Jie-lin Rong, Dan-dan Bao, Yu Zhao |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Biocompatibility viruses fungi Biomedical Engineering 02 engineering and technology General Chemistry General Medicine Transfection 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Molecular biology 0104 chemical sciences Chitosan chemistry.chemical_compound chemistry In vivo PEG ratio Gene expression Biophysics General Materials Science 0210 nano-technology Cytotoxicity Ethylene glycol |
| Zdroj: | Journal of Materials Chemistry B. 4:893-901 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c5tb02074c |
| Popis: | A core-shell structured chitosan (CS)-based gene vector with a sustainable gene transfection effect was designed and successfully prepared in this study. The pEGFP was first combined with the thiolated and N-alkylated chitosan (TACS). Then, hydroxybutyl chitosan grafted with poly(ethylene glycol) (EG-HBC) was coated on the pEGFP-loaded TACS particles. The prepared pEGFP-loaded TACS@EG-HBC particles have a size of about 200 nm and little cytotoxicity. The in vitro and in vivo gene transfection experiments indicate that the pEGFP-loaded TACS@EG-HBC particles possess a better sustainable gene transfection capacity and a high transfection efficiency, which should be attributed to the biodegradation of the CS-based shell, the thiolation and N-alkylation modification on CS cores, and the grafted PEG chains with better biocompatibility. The in vivo gene expression of the loaded pEGFP can persist up to 60 days. This novel gene vector has a theoretical and practical significance for gene therapy with sustained transfection effect. |
| Databáze: | OpenAIRE |
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