Plasmid profiles among some ESKAPE pathogens in a tertiary care centre in south India

Autor: Purva Mathur, VC Ohri, Yamuna Devi Bakthavatchalam, Agila Kumari Pragasam, Kamini Walia, Balaji Veeraraghavan, Naveen Kumar Devanga Ragupathi
Rok vydání: 2019
Předmět:
0301 basic medicine
Acinetobacter baumannii
Staphylococcus aureus
plasmids
Gene Transfer
Horizontal
Klebsiella pneumoniae
030106 microbiology
Enterococcus faecium
Enterobacter
lcsh:Medicine
India
Biology
medicine.disease_cause
Antimicrobial resistance
Genome
General Biochemistry
Genetics and Molecular Biology
beta-Lactamases
Microbiology
03 medical and health sciences
0302 clinical medicine
Plasmid
Antibiotic resistance
Drug Resistance
Multiple
Bacterial
medicine
Humans
030212 general & internal medicine
Escherichia coli
Cross Infection
Pseudomonas aeruginosa
IncF
lcsh:R
General Medicine
biology.organism_classification
β-lactamase
Anti-Bacterial Agents
Antimicrobial resistance - β-lactamase - col-horizontal gene transfer - IncF - plasmids
GenBank
Original Article
col-horizontal gene transfer
Zdroj: The Indian Journal of Medical Research
Indian Journal of Medical Research, Vol 149, Iss 2, Pp 222-231 (2019)
ISSN: 0971-5916
Popis: Background & objectives: Plasmid has led to increase in resistant bacterial pathogens through the exchange of antimicrobial resistance (AMR) genetic determinants through horizontal gene transfer. Baseline data on the occurrence of plasmids carrying AMR genes are lacking in India. This study was aimed to identify the plasmids associated with AMR genetic determinants in ESKAPE pathogens. Methods: A total of 112 ESKAPE isolates including Escherichia coli (n=37), Klebsiella pneumoniae (n=48, including 7 pan-drug susceptible isolates), Acinetobacter baumannii (n=8), Pseudomonas aeruginosa (n=1) and Staphylococcus aureus (n=18) were analyzed in the study. Isolates were screened for antimicrobial susceptibility and whole genome sequencing of isolates was performed using Ion Torrent (PGM) sequencer. Downstream data analysis was done using PATRIC, ResFinder, PlasmidFinder and MLSTFinder databases. All 88 whole genome sequences (WGS) were deposited at GenBank. Results: Most of the study isolates showed resistant phenotypes. As analyzed from WGS, the isolates included both known and unknown sequence types. The plasmid analysis revealed the presence of single or multiple plasmids in the isolates. Plasmid types such as IncHI1B(pNDM-MAR), IncFII(pRSB107), IncFIB(Mar), IncFIB(pQil), IncFIA, IncFII(K), IncR, ColKP3 and ColpVC were present in K. pneumoniae. In E. coli, IncFIA, IncFII, IncFIB, Col(BS512), IncL1, IncX3 and IncH were present along with other types. S. aureus harboured seven different plasmid groups pMW2 (rep 5), pSAS1 (rep 7), pDLK1 (rep 10), pUB110 (rep US12), Saa6159 (rep 16), pKH12 (rep 21) and pSA1308 (rep 21). The overall incidence of IncF type plasmids was 56.5 per cent followed by Col type plasmids 18.3 per cent and IncX 5.3 per cent. Other plasmid types identified were
Databáze: OpenAIRE
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