Search for novel metabolites in fungal endophytes: study of Phomopsis sp. and Colletotrichum sp. co-cultivation and Botryosphaeria mamane epigenetic modification
| Autor: | Alexis Valentin, L. Rieusset, Patricia Jargeat, Marieke Vansteelandt, C. Amasifuen, Mohamed Haddad, Fatima Barakat, Asih Triastuti, Nicolas Fabre |
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| Přispěvatelé: | Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Evolution et Diversité Biologique (EDB), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]) |
| Rok vydání: | 2016 |
| Předmět: |
Fungal endophytes
0106 biological sciences 2. Zero hunger Pharmacology new metabolite production [SDV]Life Sciences [q-bio] Organic Chemistry Pharmaceutical Science Biology 01 natural sciences Botryosphaeria mamane Analytical Chemistry Complementary and alternative medicine Colletotrichum sp 010608 biotechnology Drug Discovery Phomopsis sp Botany co-cultivation Molecular Medicine Epigenetics epigenetic 010606 plant biology & botany |
| Zdroj: | Planta Medica 9th Joint Natural Products Conference 9th Joint Natural Products Conference, Jul 2016, Copenhagen, Denmark. pp.S1-S381, ⟨10.1055/s-0036-1596716⟩ |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | International audience; Fungal endophytes are fungi that grow within plant tissues without causing immediate signs of disease. They are gaining increased attention for their ability to produce natural products with diverse structure and pharmacological activity [1]. Under conventional cultures conditions some endophytes show loss of activity in producing secondary metabolites (SM) due to inactivation of metabolite gene clusters [2]. Several strategies have been applied to activate these silent gene clusters including epigenetic modifications and co-cultivation. The signal come to the cell will modify the silence gene and lead to an enhanced production and/or to an accumulation of different compounds that are not detected in axenic cultures [3].The purpose of this study was to find new SM and analyze gene clusters activation through co-cultivation and through the addition of molecular and epigenetic modifier such as suberoylanilide hydroxamic acid or valproic acid into the culture medium. Our research group isolated 409 Ascomycetes strains from 21 medicinal plants from South America [4]. Among them, we chose Phomopsis sp. and Colletotrichum sp on the basis of ecological data as model for co-cultivation and Botrysosphaeria mamane for the epigenetic modification studies. First results obtained highlighted that the fungal co-cultivation can induce de novo synthesis of secondary metabolites (Barakat et al., data not published). In order to understand the mechanisms underlying these modifications, we focused on B. mamane, known to produce SM such as mellein, primin, botryomaman, and 4,5-dihydroxy-2-hexenoic acid[5]. This strain is being under investigation in our group for producing anti-leishmania SM. It exhibited good activity against Leishmania infantum with IC50= 17. 04 µg/ml. Investigations are currently in process to apply epigenetic modifier in order to induce SM production in this strain and analyze its metabolomics and pharmacological activity modifications against L. infantum through immunomodulatory approaches. |
| Databáze: | OpenAIRE |
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