Calcium channel subunit α2σ4 is regulated by early growth response 1 and facilitates epileptogenesis

Autor: Erick Martinez-Chavez, Arthur Bikbaev, Martin Heine, Johannes Alexander Müller, Sandra Blaess, Dirk Dietrich, Julika Pitsch, Christine K. Rummel, Albert J. Becker, Karen M.J. van Loo, Susanne Schoch
Přispěvatelé: Rehabilitation medicine
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: van Loo, K M J, Rummel, C K, Pitsch, J, Müller, J A, Bikbaev, A F, Martinez-Chavez, E, Blaess, S, Dietrich, D, Heine, M, Becker, A J & Schoch, S 2019, ' Calcium channel subunit α2σ4 is regulated by early growth response 1 and facilitates epileptogenesis ', Journal of Neuroscience, vol. 39, no. 17, pp. 3175-3187 . https://doi.org/10.1523/JNEUROSCI.1731-18.2019
Journal of Neuroscience, 39(17), 3175-3187. Society for Neuroscience
ISSN: 0270-6474
DOI: 10.1523/JNEUROSCI.1731-18.2019
Popis: Transient brain insults, including status epilepticus (SE), can trigger a period of epileptogenesis during which functional and structural reorganization of neuronal networks occurs resulting in the onset of focal epileptic seizures. In recent years, mechanisms that regulate the dynamic transcription of individual genes during epileptogenesis and thereby contribute to the development of a hyperexcitable neuronal network have been elucidated. Our own results have shown early growth response 1 (Egr1) to transiently increase expression of the T-type voltage-dependent Ca2+channel (VDCC) subunit CaV3.2, a key proepileptogenic protein. However, epileptogenesis involves complex and dynamic transcriptomic alterations; and so far, our understanding of the transcriptional control mechanism of gene regulatory networks that act in the same processes is limited. Here, we have analyzed whether Egr1 acts as a key transcriptional regulator for genes contributing to the development of hyperexcitability during epileptogenesis. We found Egr1 to drive the expression of the VDCC subunit α2δ4, which was augmented early and persistently after pilocarpine-induced SE. Furthermore, we show that increasing levels of α2δ4 in the CA1 region of the hippocampus elevate seizure susceptibility of mice by slightly decreasing local network activity. Interestingly, we also detected increased expression levels of Egr1 and α2δ4 in human hippocampal biopsies obtained from epilepsy surgery. In conclusion, Egr1 controls the abundance of the VDCC subunits CaV3.2 and α2δ4, which act synergistically in epileptogenesis, and thereby contributes to a seizure-induced “transcriptional Ca2+channelopathy.”SIGNIFICANCE STATEMENTThe onset of focal recurrent seizures often occurs after an epileptogenic process induced by transient insults to the brain. Recently, transcriptional control mechanisms for individual genes involved in converting neurons hyperexcitable have been identified, including early growth response 1 (Egr1), which activates transcription of the T-type Ca2+channel subunit CaV3.2. Here, we find Egr1 to regulate also the expression of the voltage-dependent Ca2+channel subunit α2δ4, which was augmented after pilocarpine- and kainic acid-induced status epilepticus. In addition, we observed that α2δ4 affected spontaneous network activity and the susceptibility for seizure induction. Furthermore, we detected corresponding dynamics in human biopsies from epilepsy patients. In conclusion, Egr1 orchestrates a seizure-induced “transcriptional Ca2+channelopathy” consisting of CaV3.2 and α2δ4, which act synergistically in epileptogenesis.
Databáze: OpenAIRE
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