Activity of Amphotericin B and Anidulafungin Combined with Rifampicin, Clarithromycin, Ethylenediaminetetraacetic Acid, N-Acetylcysteine, and Farnesol against Candida tropicalis Biofilms
| Autor: | Marcelo Ernesto Fernández-Rivero, Amparo Valentín, José Luis del Pozo, Araceli Molina de Diego, Javier Pemán, Emilia Cantón |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Ethylenediaminetetraacetic acid Plant Science anidulafungin Article biofilm Microbiology Candida tropicalis 03 medical and health sciences chemistry.chemical_compound Clarithromycin Amphotericin B CDC Biofilm Reactor amphotericin B PTFE titanium anti-biofilm compounds medicine Ecology Evolution Behavior and Systematics biology Biofilm Farnesol biology.organism_classification chemistry Anidulafungin Rifampicin medicine.drug Nuclear chemistry |
| Zdroj: | Journal of Fungi Journal of Fungi; Volume 3; Issue 1; Pages: 16 JOURNAL OF FUNGI r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname |
| ISSN: | 2309-608X |
| Popis: | We evaluated the activity of (1) amphotericin-B (AMB), combined with rifampicin (RIF), clarithromycin (CLA), N-acetylcysteine (NAC), ethylenediaminetetraacetic acid (EDTA), and farnesol (FAR) (1000, 1000, 1000, 4000, and 30,000 mg/L, and 300 microM, respectively), against Candida tropicalis biofilms formed on polytetrafluoroethylene (PTFE) and (2) anidulafungin (ANF) combined with the same compounds at 8, 10, 5, 40, and 30 mg/L, and 30 microM, respectively, against biofilms formed on titanium. Biofilm growth kinetics were performed in a CDC Biofilm Reactor (CBR). PTFE or titanium disks were removed from the CBR at 24, 48, 72, and 96 h to determine the Log10CFU/cm(2). Killing kinetics were performed by adding the drugs to 24-h-mature biofilms (time 0). Disks were removed after 24, 48, and 72 h of drug exposure to determine Log10CFU/cm(2). Viable cells in biofilms were 4.73 and 4.29 Log10CFU/cm(2) on PTFE and titanium, respectively. Maximum Log10 decreases in CFU/cm(2) depend on the combination and were: 3.53 (AMB + EDTA), 2.65 (AMB + RIF), 3.07 (AMB + NAC), 2.52 (AMB + CLA), 1.49 (AMB + FAR), 2.26 (ANF + EDTA), 2.45 (ANF + RIF), 2.47 (ANF + NAC), 1.52 (ANF + CLA), and 0.44 (ANF + FAR). In conclusion, EDTA, NAC, RIF, and CLA improve the activity of AMB and ANF against biofilms developed on both surfaces, which could be an effective strategy against C. tropicalis biofilm-related infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|