Isolation of genes encoding novel transcription factors which interact with the Hap complex from Aspergillus species
| Autor: | Norihiro Tsukagoshi, Tetsuo Kobayashi, Akimitsu Tanaka, Takashi Nagase, Masashi Kato |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Recombinant Fusion Proteins
Protein subunit Genes Fungal Molecular Sequence Data Saccharomyces cerevisiae Biophysics Biochemistry Fungal Proteins Structural Biology Aspergillus nidulans Genetics Amino Acid Sequence Transcription factor Gene Kluyveromyces lactis Fungal protein Base Sequence biology biology.organism_classification Yeast Aspergillus CCAAT-Binding Factor Sequence Alignment Plasmids Transcription Factors |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1576:176-182 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(02)00286-5 |
| Popis: | The Saccharomyces cerevisiae CCAAT-binding factor is composed of four subunits Hap2p, Hap3p, Hap4p and Hap5p. Three subunits, Hap2/3/5p, are required for DNA-binding and Hap4p is involved in transcriptional activation. Although homologues of Hap2/3/5p (in the case of Aspergillus nidulans; HapB/C/E, respectively) were found in many eukaryotes, no Hap4p homologues have been found except for the other yeast, Kluyveromyces lactis. With the lexA-hap2, -hapB, -hapC, or -hapE fusion gene, we evaluated the ability of interaction between Aspergillus Hap subunits and S. cerevisiae Hap4p subunit in S. cerevisiae. Using the system with lexA-hapB, a gene encoding a novel transcriptional activator, which interacted with the Hap complex, was isolated from A. nidulans and designated hapX. |
| Databáze: | OpenAIRE |
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