Identification of polyhydroxyalkanoates in Halococcus and other haloarchaeal species
| Autor: | Andrea Legat, Klaus Zangger, Claudia Gruber, Helga Stan-Lotter, Gerhard Wanner |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Halobacterium
Geologic Sediments Magnetic Resonance Spectroscopy Polyesters Halobacterium noricense ved/biology.organism_classification_rank.species Hydroxybutyrates Applied Microbiology and Biotechnology Polyhydroxyalkanoates Microbiology Halococcus 03 medical and health sciences Haloalkaliphile Microscopy Electron Transmission 030304 developmental biology Halobacteriales 0303 health sciences Halobacteriaceae biology Haloarchaea 030306 microbiology ved/biology Haloferax volcanii Australia General Medicine Halococcus dombrowskii biology.organism_classification Applied Microbial and Cell Physiology Biochemistry Halococcus salifodinae Polyhydroxybutyrate Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-010-2611-6 |
| Popis: | Polyhydroxyalkanoates (PHAs) are accumulated in many prokaryotes. Several members of the Halobacteriaceae produce poly-3-hydroxybutyrate (PHB), but it is not known if this is a general property of the family. We evaluated identification methods for PHAs with 20 haloarchaeal species, three of them isolates from Permian salt. Staining with Sudan Black B, Nile Blue A, or Nile Red was applied to screen for the presence of PHAs. Transmission electron microscopy and 1H-nuclear magnetic resonance spectroscopy were used for visualization of PHB granules and chemical confirmation of PHAs in cell extracts, respectively. We report for the first time the production of PHAs by Halococcus sp. (Halococcus morrhuae DSM 1307T, Halococcus saccharolyticus DSM 5350T, Halococcus salifodinae DSM 8989T, Halococcus dombrowskii DSM 14522T, Halococcus hamelinensis JCM 12892T, Halococcus qingdaonensis JCM 13587T), Halorubrum sp. (Hrr. coriense DSM 10284T, Halorubrum chaoviator DSM 19316T, Hrr. chaoviator strains NaxosII and AUS-1), haloalkaliphiles (Natronobacterium gregoryi NCMB 2189T, Natronococcus occultus DSM 3396T) and Halobacterium noricense DSM 9758T. No PHB was detected in Halobacterium salinarum NRC-1 ATCC 700922, Hbt. salinarum R1 and Haloferax volcanii DSM 3757T. Most species synthesized PHAs when growing in synthetic as well as in complex medium. The polyesters were generally composed of PHB and poly-ß-hydroxybutyrate-co-3-hydroxyvalerate (PHBV). Available genomic data suggest the absence of PHA synthesis in some haloarchaea and in all other Euryarchaeota and Crenarchaeota. Homologies between haloarchaeal and bacterial PHA synthesizing enzymes had indicated to some authors probable horizontal gene transfer, which, considering the data obtained in this study, may have occurred already before Permian times. Electronic supplementary material The online version of this article (doi:10.1007/s00253-010-2611-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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