Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
| Autor: | Lúcio Macedo Barbosa, Rodolfo Debone Piazza, Luis André Morais Mariúba, F S E D V Faria, C.C.V. dos Santos, Marco A. Morales, M. Zubair Iqbal, Alfredo Rodríguez, C.O. Rocha, Rodrigo Fernando Costa Marques, Yury Oliveira Chaves, Miguel Jafelicci |
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| Přispěvatelé: | Laboratory of Nanobiotechnology, Federal University of Rio Grande Do Norte, Chinese Academy of Sciences, Fundacao Oswaldo Cruz, Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
XRD
Carboxylic Acids Maghemite 02 engineering and technology 01 natural sciences Ferric Compounds chemistry.chemical_compound X-Ray Diffraction Spectroscopy Fourier Transform Infrared General Materials Science Propylamines Succinic anhydride magnetic nanobeads Silanes 021001 nanoscience & nanotechnology Magnetic Resonance Imaging Microspheres Mechanics of Materials Transmission electron microscopy Silanization Rabbits 0210 nano-technology MRI Materials science rabbit antibody Static Electricity Bioengineering engineering.material 010402 general chemistry Vibration Antibodies Fluorescence Magnetization Magnetics Spectroscopy Mossbauer Dynamic light scattering Animals Electrical and Electronic Engineering Fourier transform infrared spectroscopy Particle Size Mechanical Engineering flow cytometry mossbauer spectroscopy General Chemistry Dynamic Light Scattering 0104 chemical sciences chemistry engineering Magnetic nanoparticles Nanoparticles human activities Nuclear chemistry |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1361-6528 |
| Popis: | Made available in DSpace on 2018-12-11T16:54:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-28 Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging. Federal University of Acre Laboratory of Nanobiotechnology Federal University of Rio Grande Do Norte Department of Theoretical and Experimental Physics Division of Functional Materials and Nano-Devices Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Instituto Leonidas e Maria Deane Fundacao Oswaldo Cruz Sao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355 Sao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355 |
| Databáze: | OpenAIRE |
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