Time-related decreases in μ and δ opioid receptors in the superficial dorsal horn of the rat spinal cord following a large unilateral dorsal rhizotomy
| Autor: | M.C. Lombard, J.M. Besson, D. Besse |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.medical_treatment Population Central nervous system Receptors Opioid mu Tritium Functional Laterality chemistry.chemical_compound Reference Values Postsynaptic potential Ganglia Spinal Receptors Opioid delta Internal medicine medicine Animals Receptor education Molecular Biology Afferent Pathways education.field_of_study General Neuroscience Rhizotomy Rats Inbred Strains Enkephalins Anatomy Enkephalin Ala(2)-MePhe(4)-Gly(5) Spinal cord Rats Kinetics DAMGO medicine.anatomical_structure Endocrinology Spinal Cord Opioid chemistry Receptors Opioid Autoradiography Neurology (clinical) Spinal Nerve Roots Oligopeptides Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 578:115-121 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(92)90237-4 |
| Popis: | The aim of the present study was to measure the time-related modifications of mu and delta opioid binding sites in the superficial layers of the dorsal horn of the rat spinal cord after a C4-T2 unilateral dorsal rhizotomy. Using specific ligands, namely [3H]DAMGO for mu sites and [3H]DTLET for delta sites, and a quantitative autoradiographic analysis, we have observed: (a) a decrease in binding on the ipsilateral side to the lesion as early as the first day postrhizotomy, the maximal loss being attained at 8 days postlesion, (b) after 8 days postlesion, the residual binding remains stable over the period of analysis (90 days), (c) the loss of mu receptors (71-74%) is significantly more pronounced than the loss of delta receptors (57-62%) and (d) affinities of postsynaptic mu and delta receptors are similar to those of the total receptor population in the superficial layers of the dorsal horn. Comparison of these results with the degeneration of primary afferent fibers reported in literature favors the localization of the majority of mu and delta opioid binding sites on fine diameter primary afferent fibers. |
| Databáze: | OpenAIRE |
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