Distribution and co-occurrence of antibiotic and metal resistance genes in biofilms of an anthropogenically impacted stream
| Autor: | Jonathon B. Van Gray, Alescia A. Roberto, Jean Engohang-Ndong, Laura Gunn Leff |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
010504 meteorology & atmospheric sciences Resistance (ecology) Urban stream Stormwater Community structure Biofilm Drug Resistance Microbial 010501 environmental sciences 01 natural sciences Pollution Resistome Nutrient Rivers Genes Bacterial Metals Biofilms Environmental chemistry Environmental Chemistry Environmental science Sewage treatment Waste Management and Disposal Environmental Monitoring 0105 earth and related environmental sciences |
| Zdroj: | Science of The Total Environment. 688:437-449 |
| ISSN: | 0048-9697 |
| Popis: | Urban stream biofilms are potential hotspots for resistomes and antibiotic resistance genes (ARGs). Biofilm communities that harbor resistance genes may be influenced by contaminant input (e.g., metals and antibiotics) from urban drainage (i.e., Wastewater Treatment Plant effluent and stormwater runoff); understanding the ecology of these communities and their resistome is needed. Given the potential importance of the co-occurrence of ARGs and metal resistance genes (MRGs), we investigated the spatial and temporal distribution of three ARGs (tetracycline [tetW] and sulfonamides [sulI and sulII]), four MRGs (lead [pbrT], copper [copA], and cadmium/cobalt/zinc [czcA and czcC]) via quantitative PCR and biofilm bacterial community composition via MiSeq 16S sequencing at four time points along an urbanization gradient (i.e., developed, agriculture, and forested sites) in a stream's watershed. Our results revealed that ARG and MRG abundances were significantly affected by land use-time interaction, with greater resistance abundances occurring in more urban locations during particular times of the year. It was also observed that changes in ARG and MRG profiles were influenced by differences in community composition among land use types, and that these differences were in response to changes in stream physicochemical parameters (pH, redox, temperature, nutrient availability, and metal concentration) that were driven by sub-watershed land use. Moreover, the dynamics between ARGs and MRGs within these communities correlated strongly and positively with one another. Taken altogether, our results demonstrate that changes in environmental properties due to human activity may drive the ARG-MRG profiles of biofilm communities by modulating community structure over time and space. |
| Databáze: | OpenAIRE |
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