Invitro synergistic activity of lactic acid bacteria against multi-drug resistant staphylococci

Autor: Rama Bhadekar, Jinal Bhola
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Coagulase
Methicillin-Resistant Staphylococcus aureus
Meticillin
medicine.drug_class
Gram-positive bacteria
Antibiotic sensitivity
Antibiotics
MRSA
Microbial Sensitivity Tests
Antimicrobial activity
medicine.disease_cause
Microbiology
03 medical and health sciences
Lactic Acid Bacteria
0302 clinical medicine
Antibiotic resistance
Bacteriocins
Bacterial Proteins
Lactobacillales
Drug Resistance
Multiple
Bacterial
medicine
Humans
Multi-drug resistant
biology
Synergism
lcsh:Other systems of medicine
General Medicine
Staphylococcal Infections
lcsh:RZ201-999
biology.organism_classification
030205 complementary & alternative medicine
Anti-Bacterial Agents
Multiple drug resistance
Complementary and alternative medicine
030220 oncology & carcinogenesis
Microbial Interactions
Staphylococcus
Bacteria
medicine.drug
Research Article
Zdroj: BMC Complementary and Alternative Medicine
BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-8 (2019)
ISSN: 1472-6882
Popis: Background Multi-drug resistance in microorganisms is a serious problem at national as well as at a global level. Many researches have suggested alternatives to antibiotics with minimal or no major side effects. LAB is one of the most human-friendly probiotic strains known to mankind from times immemorial. With the objective to deal with progressing antibiotic resistance among microorganisms, the present work demonstrates the inhibitory activity of LAB consortium against MDR clinical isolates. Methods Total of nine hospital isolates of staphylococci were obtained and distinguished as S.aureus and coagulase-negative Staphylococcus (CoNS) based on their ability to ferment mannitol and form clumping with citrated plasma. All the test organisms were tested for antibiotic sensitivity with HiMedia (India) Octadisc Combi 92. Sets of L .plantarum, L .acidophilus and L.casei var. rhamnosus were prepared and tested against a standard culture of S.aureus NCIM 2129 by agar well diffusion method. To identify the primary source of substances responsible for inhibitory action, whole broth, cell-free supernatant, and cell lysate was prepared from the above-mentioned set. These were tested for their inhibitory action initially against standard S.aureus NCIM 2127, followed by clinical isolates. Results The antibiotic sensitivity profile revealed that all clinical isolates were multi-drug resistant. The maximum inhibitory potential was seen in a combination of the three LAB in the ratio 1:1:1. Highest antagonistic activity was observed with whole broth and cell lysate of LAB consortium. In liquid broth assay, the cell lysate of LAB consortium astoundingly exhibited up to 85% inhibition of multi-drug resistant Staphylococcus isolates. Conclusions Our results suggest antagonistic role of LAB metabolites against methicillin resistant staphylococci.
Databáze: OpenAIRE
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