Evaluation of Methods for Sizing and Counting of Ultrasound Contrast Agents

Autor: Petros Nihoyannopoulos, Charles A. Sennoga, Eleanor Stride, Robert J. Eckersley, Dorian O. Haskard, John M. Seddon, James S.M. Yeh, Meng-Xing Tang, Joseph V. Hajnal, Julia Alter
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Materials science
SonoVue™
Acoustics and Ultrasonics
Conductometry
Biophysics
Drug Evaluation
Preclinical
Sulfur Hexafluoride
Contrast Media
Nanotechnology
02 engineering and technology
Optical microscopy
Microbubble number count
Laser diffraction
030218 nuclear medicine & medical imaging
Microsphere
03 medical and health sciences
0302 clinical medicine
Coulter counter
Electro-impedance volumetric zone sensing
Radiology
Nuclear Medicine and imaging
Intra-method variation
Particle Size
Phospholipids
Ultrasonography
Mean diameter
Reproducibility
Radiological and Ultrasound Technology
business.industry
Ultrasound
Mean size diameter
Original Contribution
Size distribution
021001 nanoscience & nanotechnology
Sizing
Radiology Nuclear Medicine and imaging
Microbubbles
Ultrasound imaging
0210 nano-technology
business
Biomedical engineering
Zdroj: Ultrasound in Medicine & Biology
ISSN: 1879-291X
0301-5629
Popis: A precise, accurate and well documented method for the sizing and counting of microbubbles is essential for all aspects of quantitative microbubble-enhanced ultrasound imaging. The efficacy of (a) electro-impedance volumetric zone sensing (ES) also called a Coulter counter/multisizer; (b) optical microscopy (OM); and (c) laser diffraction (LD), for the sizing and counting of microbubbles was assessed. Microspheres with certified mean diameter and number concentration were used to assess sizing and counting reproducibility (precision) and reliability (accuracy) of ES, OM and LD. SonoVue™ was repeatedly (n = 3) sized and counted to validate ES, OM and LD sizing and counting efficacy. Statistical analyses of intra-method variability for the SonoVue™ mean diameter showed that the best microbubble sizing reproducibility was obtained using OM with a mean diameter sizing variability of 1.1%, compared with a variability of 4.3% for ES and 7.1% for LD. The best microbubble counting reproducibility was obtained using ES with a number concentration variability of 8.3%, compared with a variability of 22.4% for OM and 32% for LD. This study showed that no method is fully suited to both sizing and counting of microbubbles.
Databáze: OpenAIRE
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