Evaluation of Methods for Sizing and Counting of Ultrasound Contrast Agents
Autor: | Petros Nihoyannopoulos, Charles A. Sennoga, Eleanor Stride, Robert J. Eckersley, Dorian O. Haskard, John M. Seddon, James S.M. Yeh, Meng-Xing Tang, Joseph V. Hajnal, Julia Alter |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Materials science
SonoVue™ Acoustics and Ultrasonics Conductometry Biophysics Drug Evaluation Preclinical Sulfur Hexafluoride Contrast Media Nanotechnology 02 engineering and technology Optical microscopy Microbubble number count Laser diffraction 030218 nuclear medicine & medical imaging Microsphere 03 medical and health sciences 0302 clinical medicine Coulter counter Electro-impedance volumetric zone sensing Radiology Nuclear Medicine and imaging Intra-method variation Particle Size Phospholipids Ultrasonography Mean diameter Reproducibility Radiological and Ultrasound Technology business.industry Ultrasound Mean size diameter Original Contribution Size distribution 021001 nanoscience & nanotechnology Sizing Radiology Nuclear Medicine and imaging Microbubbles Ultrasound imaging 0210 nano-technology business Biomedical engineering |
Zdroj: | Ultrasound in Medicine & Biology |
ISSN: | 1879-291X 0301-5629 |
Popis: | A precise, accurate and well documented method for the sizing and counting of microbubbles is essential for all aspects of quantitative microbubble-enhanced ultrasound imaging. The efficacy of (a) electro-impedance volumetric zone sensing (ES) also called a Coulter counter/multisizer; (b) optical microscopy (OM); and (c) laser diffraction (LD), for the sizing and counting of microbubbles was assessed. Microspheres with certified mean diameter and number concentration were used to assess sizing and counting reproducibility (precision) and reliability (accuracy) of ES, OM and LD. SonoVue™ was repeatedly (n = 3) sized and counted to validate ES, OM and LD sizing and counting efficacy. Statistical analyses of intra-method variability for the SonoVue™ mean diameter showed that the best microbubble sizing reproducibility was obtained using OM with a mean diameter sizing variability of 1.1%, compared with a variability of 4.3% for ES and 7.1% for LD. The best microbubble counting reproducibility was obtained using ES with a number concentration variability of 8.3%, compared with a variability of 22.4% for OM and 32% for LD. This study showed that no method is fully suited to both sizing and counting of microbubbles. |
Databáze: | OpenAIRE |
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