Interactions between Spider Silk and Cells - NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames
| Autor: | Merlin Guggenheim, Cornelia Kasper, Joern W. Kuhbier, Bjoern Menger, Anja Hillmer, Christina Allmeling, Kerstin Reimers, Gudrun Brandes, Peter M. Vogt |
|---|---|
| Přispěvatelé: | University of Zurich |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften
Biologie lcsh:Medicine Cell morphology Mice Tissue engineering ddc:590 Biochemistry/Protein Chemistry Cell Movement Dewey Decimal Classification::500 | Naturwissenschaften::590 | Tiere (Zoologie) Spider silk Surgery/Plastic Surgery lcsh:Science 10266 Clinic for Reconstructive Surgery Biochemistry/Biomacromolecule-Ligand Interactions Multidisciplinary Tissue Scaffolds biology Nephila clavipes Biomaterial Spiders Cell Biology/Extra-Cellular Matrix Extracellular Matrix Cell biology peripheral-nerve regeneration SILK scaffolds Research Article biomaterials Cell Survival proliferation Silk Fibroin 610 Medicine & health 1100 General Agricultural and Biological Sciences macromolecular substances in-vitro fibers clavipes dragline silk nephila-clavipes complex mixtures 1300 General Biochemistry Genetics and Molecular Biology ddc:570 Cell Adhesion Animals Cell Proliferation 1000 Multidisciplinary Spider lcsh:R fungi technology industry and agriculture Fibroblasts biology.organism_classification equipment and supplies fibroin proteins Cell Biology/Cell Adhesion 10022 Division of Surgical Research NIH 3T3 Cells lcsh:Q |
| Zdroj: | PLoS ONE 5 (2010), Nr. 8 PLoS ONE PLoS ONE, Vol 5, Iss 8, p e12032 (2010) |
| Popis: | Background: Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could be spider silk. Not only does it own unique mechanical properties, its biocompatibility has been shown already in vivo. In our study, we used native spider dragline silk which is known as the strongest fibre in nature. Methodology/Principal Findings: Steel frames were originally designed and manufactured and woven with spider silk, harvesting dragline silk directly out of the animal. After sterilization, scaffolds were seeded with fibroblasts to analyse cell proliferation and adhesion. Analysis of cell morphology and actin filament alignment clearly revealed adherence. Proliferation was measured by cell count as well as determination of relative fluorescence each after 1, 2, 3, and 5 days. Cell counts for native spider silk were also compared with those for trypsin-digested spider silk. Spider silk specimens displayed less proliferation than collagen-and fibronectin-coated cover slips, enzymatic treatment reduced adhesion and proliferation rates tendentially though not significantly. Nevertheless, proliferation could be proven with high significance (p |
| Databáze: | OpenAIRE |
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