Distribution of Select Cement Proteins in the Acorn Barnacle Amphibalanus amphitrite
Autor: | Gary H. Dickinson, Jenifer M. Scancella, Janna N. Schultzhaus, Chris R. Taitt, Chenyue Wang, Dagmar H. Leary, Shrey Patel, Daniel A. Phillips, Madeline Smerchansky, Christopher M. Spillmann, Kathryn J. Wahl, W. Judson Hervey, Christopher R. So |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
barnacle cement 010504 meteorology & atmospheric sciences lcsh:QH1-199.5 Ocean Engineering Aquatic Science lcsh:General. Including nature conservation geographical distribution Oceanography Proteomics confocal microscopy 01 natural sciences Barnacle lcsh:Science cement protein Organism 0105 earth and related environmental sciences Water Science and Technology mass spectrometry Cement Global and Planetary Change biology acorn barnacle Chemistry 010604 marine biology & hydrobiology tissue sections Substrate (biology) biology.organism_classification Amphibalanus amphitrite Biophysics lcsh:Q Adhesive Immunostaining |
Zdroj: | Frontiers in Marine Science, Vol 7 (2020) |
ISSN: | 2296-7745 |
Popis: | Acorn barnacles are major marine fouling organisms. Their success is largely due to an ability to adhere to diverse substrates via a sub-micron thick proteinaceous adhesive layer that develops as the organism molts and expands its base. Recent work has expanded the set of proteins identified within the adhesive interface, but one outstanding question concerns their spatial distribution throughout the organism. Here, we employ immunological analysis of Amphibalanus amphitrite tissue sections and identify the presence of two cement proteins, AaCP19-1 and AaCP43-1, in areas far removed from the adhesive interface. Confocal imaging reveals specific staining along different tissue linings of the organism as well as other non-cementing regions. Additionally, we employ a modified, pressure cycling technology approach to recover protein from histological tissue sections to perform proteomics analysis. Mass spectrometry analysis of proteins recovered from transverse histological sections of the upper portion of barnacles indicates the presence of these same proteins, complementing the immunostaining observations. The proteomics analysis also revealed the presence of other proteins first identified in the adhesive layer. While some proteins are clearly enriched at the surface interface, our findings challenge the concept that cement proteins are exclusive to the substrate interface and suggest they may have an expanded physiological role beyond substrate adhesion-related processes of A. amphitrite. |
Databáze: | OpenAIRE |
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